Detailed information of TA system TA079401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 485456..486129
• Replicon: plasmid unnamed1
• Accession: NZ_CP022605
• Organism: [Ochrobactrum] quorumnocens strain A44

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CES85_RS24475
• Protein ID: WP_095448407.1
• Coordinates: 485722..486129 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CES85_RS24470
• Protein ID: WP_095448406.1
• Coordinates: 485456..485725 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CES85_RS24420	480502..480711	+	210	WP_095448400.1	hypothetical protein	-
CES85_RS24425	480730..481698	+	969	WP_095448401.1	zinc-binding dehydrogenase	-
CES85_RS24430	481743..482155	-	413	Protein_453	biotin/lipoyl-binding protein	-
CES85_RS24440	482379..482570	-	192	WP_095448402.1	hypothetical protein	-
CES85_RS24445	482667..483017	-	351	WP_095448403.1	hypothetical protein	-
CES85_RS24450	483134..483547	-	414	WP_095448404.1	type II toxin-antitoxin system VapC family toxin	-
CES85_RS24455	483544..483792	-	249	WP_095448405.1	plasmid stabilization protein	-
CES85_RS24465	484150..485364	+	1215	WP_157743551.1	IS4 family transposase	-
CES85_RS24470	485456..485725	+	270	WP_095448406.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CES85_RS24475	485722..486129	+	408	WP_095448407.1	type II toxin-antitoxin system VapC family toxin	Toxin
CES85_RS24480	486312..488114	+	1803	WP_095448408.1	DNA helicase RecQ	-
CES85_RS24485	488454..489164	+	711	WP_095448409.1	IS6 family transposase	-
CES85_RS24490	489140..489820	-	681	WP_095448410.1	Flp pilus assembly complex ATPase component TadA	-
CES85_RS24495	490324..490575	+	252	WP_157743506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..1032012	1032012
	-	flank	IS/Tn	-	-	488454..489164	710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14392.63 Da
• Isoelectric Point: 6.7118

>T79401 WP_095448407.1 NZ_CP022605:485722-486129 [[Ochrobactrum] quorumnocens]
VSALYMLDTNVVSELARNPKGAVARRIAEVGPEAVCVSIITAAELHYGCAKKGSPKLLAHIEAILGSMQVLALDTPGDAE
YGRIRAELEAVGRPIGPNDLFIAAHAYALGVVLVTANTGEFSRVRALRVENWLRD

Nucleotide

• Length: 408 bp

>T79401 NZ_CP022605:485722-486129 [[Ochrobactrum] quorumnocens]
GTGAGCGCGCTCTACATGCTTGACACCAATGTCGTGTCCGAGCTTGCACGAAACCCCAAAGGTGCTGTCGCCAGACGCAT
CGCCGAGGTTGGACCAGAAGCAGTTTGTGTCAGTATCATCACGGCAGCAGAGTTGCATTATGGATGCGCCAAAAAGGGGT
CGCCGAAGCTACTGGCGCACATCGAGGCCATTCTTGGAAGTATGCAAGTGCTCGCACTTGATACGCCTGGTGACGCCGAA
TATGGGCGAATCCGAGCCGAATTGGAAGCCGTTGGTAGGCCCATCGGTCCAAATGACCTCTTCATCGCTGCCCATGCTTA
TGCGCTCGGGGTGGTGTTAGTCACGGCCAATACCGGAGAATTCTCACGCGTCCGTGCTCTGAGGGTCGAAAACTGGCTAC
GCGACTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10171.72 Da
• Isoelectric Point: 4.8552

>AT79401 WP_095448406.1 NZ_CP022605:485456-485725 [[Ochrobactrum] quorumnocens]
MPQLRHSEPTPAREAKLFRNNKSQAVRIPADFELPGDRVMIHRDGDRLIIEPVRRLNLLEVLAGLEPLGPEDQFPNIDDT
LLPLKEIDL

Nucleotide

• Length: 270 bp

>AT79401 NZ_CP022605:485456-485725 [[Ochrobactrum] quorumnocens]
ATGCCACAGCTCCGACACTCCGAACCTACACCAGCCCGCGAAGCAAAACTCTTCCGTAACAATAAGAGTCAGGCTGTACG
CATTCCAGCTGACTTCGAACTTCCCGGCGACCGGGTGATGATCCATCGCGATGGAGATCGACTTATCATAGAGCCGGTGC
GTCGCCTGAACCTGTTGGAAGTGCTTGCCGGATTGGAACCGCTTGGGCCGGAAGATCAGTTCCCTAATATTGACGATACC
CTGCTACCGTTGAAAGAAATCGACCTGTGA