Detailed information of TA system TA079400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 476235..476866
• Replicon: plasmid unnamed1
• Accession: NZ_CP022605
• Organism: [Ochrobactrum] quorumnocens strain A44

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CES85_RS24390
• Protein ID: WP_095448394.1
• Coordinates: 476235..476636 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CES85_RS24395
• Protein ID: WP_095448395.1
• Coordinates: 476636..476866 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CES85_RS24355	472238..472903	-	666	WP_157743503.1	helix-turn-helix domain-containing protein	-
CES85_RS24360	473278..474059	+	782	Protein_440	IS701 family transposase	-
CES85_RS24370	474308..474697	+	390	WP_095444932.1	transposase	-
CES85_RS24375	474688..475089	+	402	WP_084486908.1	IS5 family transposase	-
CES85_RS24380	475088..475699	+	612	Protein_443	IS6 family transposase	-
CES85_RS24385	475742..475990	+	249	WP_157743504.1	IS3 family transposase	-
CES85_RS24390	476235..476636	-	402	WP_095448394.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CES85_RS24395	476636..476866	-	231	WP_095448395.1	antitoxin	Antitoxin
CES85_RS24400	477253..477939	-	687	WP_095448396.1	IS6 family transposase	-
CES85_RS24405	478179..478532	-	354	WP_157743505.1	ATP-binding cassette domain-containing protein	-
CES85_RS24410	478724..479617	-	894	WP_095448398.1	LysR family transcriptional regulator	-
CES85_RS24415	479771..480415	+	645	WP_095448399.1	hydrolase	-
CES85_RS24420	480502..480711	+	210	WP_095448400.1	hypothetical protein	-
CES85_RS24425	480730..481698	+	969	WP_095448401.1	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	477253..477939	686
	-	inside	Mobilizable plasmid	-	-	1..1032012	1032012

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15098.26 Da
• Isoelectric Point: 7.3839

>T79400 WP_095448394.1 NZ_CP022605:c476636-476235 [[Ochrobactrum] quorumnocens]
MLKYMLDTNICIFTIKNRPQQVREAFNRLHDQLCISSVSLMELIYGAEKSHHPEKNLAVVEGFAARLEVLSYDEPAANHT
GQLRAELARNGTPIGPYDQLIAGHARSRGLIIVTNNRREFDRVPGLRVEDWTV

Nucleotide

• Length: 402 bp

>T79400 NZ_CP022605:c476636-476235 [[Ochrobactrum] quorumnocens]
ATGCTGAAGTACATGCTGGACACCAATATATGTATTTTTACGATCAAGAATCGACCGCAGCAAGTGCGTGAGGCGTTCAA
CCGCCTGCACGATCAATTGTGCATCAGCTCGGTTAGTTTGATGGAATTAATTTACGGTGCGGAAAAGTCTCACCATCCGG
AAAAGAACCTTGCCGTCGTGGAGGGGTTCGCAGCTCGTCTTGAGGTATTATCATACGATGAACCGGCGGCAAACCATACG
GGTCAATTACGTGCCGAATTGGCACGCAATGGAACTCCTATCGGCCCATACGATCAGTTGATTGCTGGCCACGCTCGCTC
ACGTGGCCTGATTATAGTTACGAATAATCGCAGGGAGTTTGATCGTGTTCCTGGTTTACGAGTAGAAGACTGGACTGTTT
AG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8475.56 Da
• Isoelectric Point: 4.6721

>AT79400 WP_095448395.1 NZ_CP022605:c476866-476636 [[Ochrobactrum] quorumnocens]
MEQGAVFQSNRSQAIRLPKAVALPADVKRVDIVAVGRTRIIAPAGEVWDSWFDGLEVTSDFMNDRVQPASQERETF

Nucleotide

• Length: 231 bp

>AT79400 NZ_CP022605:c476866-476636 [[Ochrobactrum] quorumnocens]
ATGGAGCAGGGAGCGGTATTCCAGAGCAACCGAAGCCAAGCTATTCGCTTGCCGAAGGCAGTAGCTTTGCCTGCTGATGT
GAAGCGTGTCGATATCGTTGCTGTGGGGCGCACTCGAATTATAGCGCCTGCTGGCGAGGTTTGGGATAGCTGGTTTGACG
GCTTAGAGGTGACGTCCGACTTCATGAATGATCGTGTTCAGCCTGCGTCGCAGGAACGCGAGACGTTTTAA