Detailed information of TA system TA079381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2224619..2225148
• Replicon: chromosome
• Accession: NZ_CP022582
• Organism: Staphylococcus aureus strain FORC_062

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FORC62_RS11670
• Protein ID: WP_000621175.1
• Coordinates: 2224619..2224981 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FORC62_RS11675
• Protein ID: WP_000948331.1
• Coordinates: 2224978..2225148 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC62_RS11645	2221598..2222368	-	771	WP_001041106.1	RNA polymerase sigma factor SigB	-
FORC62_RS11650	2222343..2222822	-	480	WP_044421339.1	anti-sigma B factor RsbW	-
FORC62_RS11655	2222824..2223150	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FORC62_RS11660	2223269..2224270	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FORC62_RS11670	2224619..2224981	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FORC62_RS11675	2224978..2225148	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FORC62_RS11680	2225233..2226381	-	1149	WP_001281154.1	alanine racemase	-
FORC62_RS11685	2226447..2226806	-	360	WP_000581197.1	holo-ACP synthase	-
FORC62_RS11690	2226810..2227301	-	492	WP_001205912.1	PH domain-containing protein	-
FORC62_RS11695	2227288..2228871	-	1584	WP_001294626.1	PH domain-containing protein	-
FORC62_RS11700	2228864..2229343	-	480	WP_001287087.1	hypothetical protein	-
FORC62_RS11705	2229551..2230111	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T79381 WP_000621175.1 NZ_CP022582:c2224981-2224619 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T79381 NZ_CP022582:c2224981-2224619 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT79381 WP_000948331.1 NZ_CP022582:c2225148-2224978 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT79381 NZ_CP022582:c2225148-2224978 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1