Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 4257692..4258366 Replicon chromosome
Accession NZ_CP022578
Organism Mycobacterium tuberculosis strain WC059

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag CHR52_RS20785 Protein ID WP_003417282.1
Coordinates 4257938..4258366 (+) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag CHR52_RS20780 Protein ID WP_003417286.1
Coordinates 4257692..4257934 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHR52_RS20755 4254228..4255292 + 1065 WP_003913037.1 GTP 3',8-cyclase MoaA -
CHR52_RS20760 4255389..4255763 + 375 WP_003417295.1 4a-hydroxytetrahydrobiopterin dehydratase -
CHR52_RS20765 4255760..4256293 + 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
CHR52_RS20770 4256294..4256959 + 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
CHR52_RS20775 4256956..4257570 + 615 WP_003417288.1 class I SAM-dependent methyltransferase -
CHR52_RS20780 4257692..4257934 + 243 WP_003417286.1 ribbon-helix-helix domain-containing protein Antitoxin
CHR52_RS20785 4257938..4258366 + 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
CHR52_RS20790 4258445..4259236 - 792 WP_003417279.1 succinate dehydrogenase iron-sulfur subunit -
CHR52_RS20795 4259236..4261008 - 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
CHR52_RS20800 4261137..4261571 - 435 WP_003902441.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
CHR52_RS20805 4261568..4262002 - 435 WP_003900017.1 succinate dehydrogenase, cytochrome b556 subunit -
CHR52_RS20810 4262143..4262544 + 402 WP_003417264.1 cytidine deaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T79372 WP_003417282.1 NZ_CP022578:4257938-4258366 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T79372 NZ_CP022578:4257938-4258366 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT79372 WP_003417286.1 NZ_CP022578:4257692-4257934 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT79372 NZ_CP022578:4257692-4257934 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References