Detailed information of TA system TA079371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 4191112..4191641
• Replicon: chromosome
• Accession: NZ_CP022578
• Organism: Mycobacterium tuberculosis strain WC059

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: CHR52_RS20525
• Protein ID: WP_003417760.1
• Coordinates: 4191112..4191369 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: CHR52_RS20530
• Protein ID: WP_003417757.1
• Coordinates: 4191366..4191641 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHR52_RS20495	4187540..4187932	+	393	WP_003417776.1	roadblock/LC7 domain-containing protein	-
CHR52_RS20500	4187910..4188278	+	369	WP_003417772.1	DUF742 domain-containing protein	-
CHR52_RS20505	4188259..4188840	+	582	WP_003417769.1	ATP/GTP-binding protein	-
CHR52_RS20510	4188847..4189398	+	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
CHR52_RS20515	4189395..4189763	-	369	WP_003417765.1	FHA domain-containing protein	-
CHR52_RS20520	4189880..4191070	-	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
CHR52_RS20525	4191112..4191369	-	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
CHR52_RS20530	4191366..4191641	-	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
CHR52_RS20535	4191765..4192610	+	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
CHR52_RS20540	4192607..4192900	+	294	WP_003417749.1	DUF3017 domain-containing protein	-
CHR52_RS20545	4192914..4193303	-	390	WP_003417745.1	DUF732 domain-containing protein	-
CHR52_RS20550	4193418..4193678	+	261	WP_003417741.1	hypothetical protein	-
CHR52_RS21710	4193705..4193923	+	219	WP_157132559.1	hypothetical protein	-
CHR52_RS20560	4193821..4194192	+	372	WP_003417739.1	FAD-binding oxidoreductase	-
CHR52_RS20565	4194274..4195068	+	795	WP_003417738.1	BBE domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T79371 WP_003417760.1 NZ_CP022578:c4191369-4191112 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T79371 NZ_CP022578:c4191369-4191112 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT79371 WP_003417757.1 NZ_CP022578:c4191641-4191366 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT79371 NZ_CP022578:c4191641-4191366 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• PDB: 3CTO
• PDB: 3OEI
• PDB: 3D55
• AlphaFold DB: G0TI94