Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1856923..1857539 Replicon chromosome
Accession NZ_CP022578
Organism Mycobacterium tuberculosis strain WC059

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag CHR52_RS09195 Protein ID WP_003407593.1
Coordinates 1856923..1857240 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag CHR52_RS09200 Protein ID WP_003900349.1
Coordinates 1857237..1857539 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHR52_RS09165 1851932..1852960 - 1029 WP_003407612.1 glycosyltransferase family 2 protein -
CHR52_RS21790 1853230..1853454 + 225 WP_160522526.1 NUDIX domain-containing protein -
CHR52_RS09175 1853464..1853676 + 213 WP_003898900.1 dodecin family protein -
CHR52_RS09180 1853872..1854507 + 636 WP_071854212.1 class I SAM-dependent methyltransferase -
CHR52_RS09185 1854580..1855869 - 1290 WP_003902090.1 serine hydrolase -
CHR52_RS09190 1855922..1856926 - 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
CHR52_RS09195 1856923..1857240 - 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
CHR52_RS09200 1857237..1857539 - 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
CHR52_RS09205 1857553..1859805 - 2253 WP_003407587.1 methylmalonyl-CoA mutase -
CHR52_RS09210 1859806..1861653 - 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T79342 WP_003407593.1 NZ_CP022578:c1857240-1856923 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T79342 NZ_CP022578:c1857240-1856923 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT79342 WP_003900349.1 NZ_CP022578:c1857539-1857237 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT79342 NZ_CP022578:c1857539-1857237 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References