Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1604212..1604825 Replicon chromosome
Accession NZ_CP022578
Organism Mycobacterium tuberculosis strain WC059

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag CHR52_RS08070 Protein ID WP_003408465.1
Coordinates 1604436..1604825 (+) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag CHR52_RS08065 Protein ID WP_003408469.1
Coordinates 1604212..1604439 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHR52_RS08045 1600104..1600814 + 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -
CHR52_RS08050 1600804..1601223 + 420 WP_003408483.1 hypothetical protein -
CHR52_RS08055 1601266..1602513 - 1248 WP_003408476.1 serine hydrolase -
CHR52_RS08060 1602510..1603994 - 1485 WP_003408473.1 carboxylase -
CHR52_RS08065 1604212..1604439 + 228 WP_003408469.1 antitoxin Antitoxin
CHR52_RS08070 1604436..1604825 + 390 WP_003408465.1 PIN domain-containing protein Toxin
CHR52_RS08080 1605435..1606214 - 780 WP_003408460.1 IclR family transcriptional regulator -
CHR52_RS08085 1606228..1607046 - 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
CHR52_RS08090 1607099..1607449 - 351 WP_003898986.1 cupin domain-containing protein -
CHR52_RS08095 1607449..1608279 - 831 WP_003408448.1 cyclase family protein -
CHR52_RS08100 1608282..1609196 - 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T79340 WP_003408465.1 NZ_CP022578:1604436-1604825 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T79340 NZ_CP022578:1604436-1604825 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT79340 WP_003408469.1 NZ_CP022578:1604212-1604439 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT79340 NZ_CP022578:1604212-1604439 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References