Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1296340..1296981 Replicon chromosome
Accession NZ_CP022578
Organism Mycobacterium tuberculosis strain WC059

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag CHR52_RS06495 Protein ID WP_003410075.1
Coordinates 1296340..1296738 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WLM7
Locus tag CHR52_RS06500 Protein ID WP_003899127.1
Coordinates 1296739..1296981 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHR52_RS06445 1291346..1291621 - 276 WP_016330411.1 hypothetical protein -
CHR52_RS06455 1291939..1293195 + 1257 WP_003902247.1 HNH endonuclease -
CHR52_RS06460 1293323..1293913 - 591 WP_003899131.1 IS110 family transposase -
CHR52_RS06465 1293867..1294514 - 648 WP_071854215.1 transposase -
CHR52_RS06470 1294575..1294754 + 180 Protein_1225 hypothetical protein -
CHR52_RS06485 1295191..1295685 - 495 WP_003410080.1 DUF1398 domain-containing protein -
CHR52_RS06490 1295726..1296157 + 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
CHR52_RS06495 1296340..1296738 - 399 WP_003410075.1 PIN domain nuclease Toxin
CHR52_RS06500 1296739..1296981 - 243 WP_003899127.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CHR52_RS06505 1297069..1298394 + 1326 WP_003410070.1 DUF4143 domain-containing protein -
CHR52_RS06510 1298583..1298927 + 345 WP_003410065.1 ferredoxin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(7-48)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T79332 WP_003410075.1 NZ_CP022578:c1296738-1296340 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T79332 NZ_CP022578:c1296738-1296340 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8851.73 Da        Isoelectric Point: 4.1621

>AT79332 WP_003899127.1 NZ_CP022578:c1296981-1296739 [Mycobacterium tuberculosis]
VYSGVVSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 243 bp

>AT79332 NZ_CP022578:c1296981-1296739 [Mycobacterium tuberculosis]
GTGTATAGTGGTGTTGTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACT
CGATTCCAAGCGAAGTGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGC
TGCAGGGCAGCGGTTTCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCG
TAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
PDB 4CHG
AlphaFold DB A0A045JPF9

References