Detailed information of TA system TA079326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 696843..697483
• Replicon: chromosome
• Accession: NZ_CP022578
• Organism: Mycobacterium tuberculosis strain WC059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: CHR52_RS03580
• Protein ID: WP_003412970.1
• Coordinates: 697064..697483 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: CHR52_RS03575
• Protein ID: WP_003412975.1
• Coordinates: 696843..697067 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHR52_RS03555	692460..693023	+	564	WP_003412989.1	elongation factor P	-
CHR52_RS03560	693026..693496	+	471	WP_003899364.1	transcription antitermination factor NusB	-
CHR52_RS03565	693496..693897	+	402	WP_003412981.1	hypothetical protein	-
CHR52_RS03570	693969..696812	+	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
CHR52_RS03575	696843..697067	+	225	WP_003412975.1	antitoxin VapB39	Antitoxin
CHR52_RS03580	697064..697483	+	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
CHR52_RS03585	697484..698434	-	951	WP_003911916.1	Lsr2 family protein	-
CHR52_RS21740	698493..698738	+	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CHR52_RS03590	699079..699999	+	921	WP_003412965.1	restriction endonuclease	-
CHR52_RS03595	700034..700435	-	402	WP_003412963.1	PIN domain-containing protein	-
CHR52_RS03600	700432..700654	-	223	Protein_683	type II toxin-antitoxin system VapB family antitoxin	-
CHR52_RS03610	701171..701893	+	723	WP_003412957.1	DUF1906 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T79326 WP_003412970.1 NZ_CP022578:697064-697483 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T79326 NZ_CP022578:697064-697483 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT79326 WP_003412975.1 NZ_CP022578:696843-697067 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT79326 NZ_CP022578:696843-697067 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09