Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 681387..682024 Replicon chromosome
Accession NZ_CP022578
Organism Mycobacterium tuberculosis strain WC059

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag CHR52_RS03470 Protein ID WP_003413180.1
Coordinates 681629..682024 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag CHR52_RS03465 Protein ID WP_003413183.1
Coordinates 681387..681632 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHR52_RS03440 677981..678493 + 513 WP_003413197.1 Holliday junction resolvase RuvX -
CHR52_RS03445 678486..679739 + 1254 WP_003413196.1 endolytic transglycosylase MltG -
CHR52_RS03450 679736..680545 + 810 WP_003413193.1 shikimate dehydrogenase -
CHR52_RS03455 680557..680976 + 420 WP_003413190.1 A24 family peptidase -
CHR52_RS03465 681387..681632 + 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
CHR52_RS03470 681629..682024 + 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
CHR52_RS03475 682124..682498 + 375 WP_003413177.1 hypothetical protein -
CHR52_RS03480 682514..682891 - 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
CHR52_RS03485 682888..683145 - 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
CHR52_RS03490 683184..683597 - 414 WP_003413164.1 PIN domain nuclease -
CHR52_RS03495 683690..683967 - 278 Protein_662 type II toxin-antitoxin system VapB family antitoxin -
CHR52_RS03500 683964..684626 - 663 WP_003905878.1 hypothetical protein -
CHR52_RS03505 684623..685282 - 660 WP_003902296.1 hypothetical protein -
CHR52_RS03515 685409..686620 - 1212 WP_003900845.1 alpha/beta hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T79323 WP_003413180.1 NZ_CP022578:681629-682024 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T79323 NZ_CP022578:681629-682024 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT79323 WP_003413183.1 NZ_CP022578:681387-681632 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT79323 NZ_CP022578:681387-681632 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References