Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 440774..441344 Replicon chromosome
Accession NZ_CP022578
Organism Mycobacterium tuberculosis strain WC059

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag CHR52_RS02110 Protein ID WP_003414166.1
Coordinates 440988..441344 (+) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag CHR52_RS02105 Protein ID WP_003901465.1
Coordinates 440774..441004 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHR52_RS02065 435792..436103 - 312 WP_003414190.1 hypothetical protein -
CHR52_RS02070 436208..436465 - 258 WP_003899489.1 hypothetical protein -
CHR52_RS02075 436699..437853 - 1155 WP_003899488.1 DDE-type integrase/transposase/recombinase -
CHR52_RS02080 438052..438243 - 192 WP_003414184.1 hypothetical protein -
CHR52_RS02085 438240..438644 - 405 WP_003414181.1 hypothetical protein -
CHR52_RS21655 438792..439046 + 255 WP_003917684.1 hypothetical protein -
CHR52_RS02095 439222..439497 - 276 WP_003911993.1 DUF1778 domain-containing protein -
CHR52_RS02100 439688..440731 + 1044 WP_003414172.1 DUF2293 domain-containing protein -
CHR52_RS02105 440774..441004 + 231 WP_003901465.1 antitoxin MazE Antitoxin
CHR52_RS02110 440988..441344 + 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
CHR52_RS02115 441446..443095 - 1650 WP_003899485.1 CocE/NonD family hydrolase -
CHR52_RS02120 443114..443743 - 630 WP_003414158.1 DUF3558 family protein -
CHR52_RS02125 443874..444200 + 327 WP_003414157.1 hypothetical protein -
CHR52_RS02130 444204..445892 + 1689 WP_003414155.1 alpha/beta hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 429886..470248 40362


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T79318 WP_003414166.1 NZ_CP022578:440988-441344 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T79318 NZ_CP022578:440988-441344 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT79318 WP_003901465.1 NZ_CP022578:440774-441004 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT79318 NZ_CP022578:440774-441004 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References