Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 4402196..4402870 Replicon chromosome
Accession NZ_CP022577
Organism Mycobacterium tuberculosis strain WC078

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag CHR51_RS21490 Protein ID WP_003417282.1
Coordinates 4402442..4402870 (+) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag CHR51_RS21485 Protein ID WP_003417286.1
Coordinates 4402196..4402438 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHR51_RS21460 4398732..4399796 + 1065 WP_003913037.1 GTP 3',8-cyclase MoaA -
CHR51_RS21465 4399893..4400267 + 375 WP_003417295.1 4a-hydroxytetrahydrobiopterin dehydratase -
CHR51_RS21470 4400264..4400797 + 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
CHR51_RS21475 4400798..4401463 + 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
CHR51_RS21480 4401460..4402074 + 615 WP_003417288.1 class I SAM-dependent methyltransferase -
CHR51_RS21485 4402196..4402438 + 243 WP_003417286.1 ribbon-helix-helix domain-containing protein Antitoxin
CHR51_RS21490 4402442..4402870 + 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
CHR51_RS21495 4402949..4403740 - 792 WP_003417279.1 succinate dehydrogenase iron-sulfur subunit -
CHR51_RS21500 4403740..4405512 - 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
CHR51_RS21505 4405641..4406075 - 435 WP_003902441.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
CHR51_RS21510 4406072..4406506 - 435 WP_003900017.1 succinate dehydrogenase, cytochrome b556 subunit -
CHR51_RS21515 4406647..4407048 + 402 WP_003417264.1 cytidine deaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T79313 WP_003417282.1 NZ_CP022577:4402442-4402870 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T79313 NZ_CP022577:4402442-4402870 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT79313 WP_003417286.1 NZ_CP022577:4402196-4402438 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT79313 NZ_CP022577:4402196-4402438 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References