Detailed information of TA system TA079312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 4335616..4336145
• Replicon: chromosome
• Accession: NZ_CP022577
• Organism: Mycobacterium tuberculosis strain WC078

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: CHR51_RS21230
• Protein ID: WP_003417760.1
• Coordinates: 4335616..4335873 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: CHR51_RS21235
• Protein ID: WP_003417757.1
• Coordinates: 4335870..4336145 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHR51_RS21200	4332044..4332436	+	393	WP_003417776.1	roadblock/LC7 domain-containing protein	-
CHR51_RS21205	4332414..4332782	+	369	WP_003417772.1	DUF742 domain-containing protein	-
CHR51_RS21210	4332763..4333344	+	582	WP_003417769.1	ATP/GTP-binding protein	-
CHR51_RS21215	4333351..4333902	+	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
CHR51_RS21220	4333899..4334267	-	369	WP_003417765.1	FHA domain-containing protein	-
CHR51_RS21225	4334384..4335574	-	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
CHR51_RS21230	4335616..4335873	-	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
CHR51_RS21235	4335870..4336145	-	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
CHR51_RS21240	4336269..4337114	+	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
CHR51_RS21245	4337111..4337404	+	294	WP_003417749.1	DUF3017 domain-containing protein	-
CHR51_RS21250	4337418..4337807	-	390	WP_003417745.1	DUF732 domain-containing protein	-
CHR51_RS21255	4337922..4338182	+	261	WP_003417741.1	hypothetical protein	-
CHR51_RS21710	4338209..4338427	+	219	WP_157132559.1	hypothetical protein	-
CHR51_RS21265	4338325..4338696	+	372	WP_003417739.1	FAD-binding oxidoreductase	-
CHR51_RS21270	4338778..4339572	+	795	WP_003417738.1	BBE domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T79312 WP_003417760.1 NZ_CP022577:c4335873-4335616 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T79312 NZ_CP022577:c4335873-4335616 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT79312 WP_003417757.1 NZ_CP022577:c4336145-4335870 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT79312 NZ_CP022577:c4336145-4335870 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• PDB: 3CTO
• PDB: 3OEI
• PDB: 3D55
• AlphaFold DB: G0TI94