Detailed information of TA system TA079306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 3329101..3329672
• Replicon: chromosome
• Accession: NZ_CP022577
• Organism: Mycobacterium tuberculosis strain WC078

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: CHR51_RS16530
• Protein ID: WP_003401560.1
• Coordinates: 3329370..3329672 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: CHR51_RS16525
• Protein ID: WP_003401563.1
• Coordinates: 3329101..3329322 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHR51_RS16510	3327006..3327914	-	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-
CHR51_RS16515	3327911..3328543	-	633	WP_003905275.1	TetR/AcrR family transcriptional regulator	-
CHR51_RS16520	3328679..3329104	-	426	WP_003401566.1	PIN domain nuclease	-
CHR51_RS16525	3329101..3329322	-	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
CHR51_RS16530	3329370..3329672	-	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
CHR51_RS16535	3329669..3329896	-	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
CHR51_RS16540	3330039..3331814	-	1776	WP_010886074.1	PE family protein	-
CHR51_RS16545	3331993..3333390	+	1398	WP_003401544.1	sulfatase	-
CHR51_RS16550	3333400..3334203	+	804	WP_003401540.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T79306 WP_003401560.1 NZ_CP022577:c3329672-3329370 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T79306 NZ_CP022577:c3329672-3329370 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT79306 WP_003401563.1 NZ_CP022577:c3329322-3329101 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT79306 NZ_CP022577:c3329322-3329101 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48