Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2461636..2462267 Replicon chromosome
Accession NZ_CP022577
Organism Mycobacterium tuberculosis strain WC078

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag CHR51_RS12090 Protein ID WP_003405820.1
Coordinates 2461956..2462267 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag CHR51_RS12085 Protein ID WP_003405836.1
Coordinates 2461636..2461956 (+) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHR51_RS12060 2456832..2457470 + 639 WP_003898730.1 lipid droplet-associated protein -
CHR51_RS12065 2457467..2458714 + 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
CHR51_RS12070 2458704..2458961 + 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
CHR51_RS12075 2458971..2460083 + 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
CHR51_RS12080 2460101..2461626 - 1526 Protein_2289 carboxylesterase/lipase family protein -
CHR51_RS12085 2461636..2461956 + 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
CHR51_RS12090 2461956..2462267 + 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
CHR51_RS12095 2462379..2463536 + 1158 WP_003898727.1 AI-2E family transporter -
CHR51_RS12100 2463543..2464244 - 702 WP_003405815.1 DUF4245 domain-containing protein -
CHR51_RS12105 2464243..2465331 + 1089 WP_003898726.1 class II fructose-bisphosphatase -
CHR51_RS12110 2465362..2466786 + 1425 WP_003405805.1 class II fumarate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T79288 WP_003405820.1 NZ_CP022577:2461956-2462267 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T79288 NZ_CP022577:2461956-2462267 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT79288 WP_003405836.1 NZ_CP022577:2461636-2461956 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT79288 NZ_CP022577:2461636-2461956 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References