Detailed information of TA system TA079287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2452943..2453511
• Replicon: chromosome
• Accession: NZ_CP022577
• Organism: Mycobacterium tuberculosis strain WC078

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: CHR51_RS12035
• Protein ID: WP_003405865.1
• Coordinates: 2452943..2453317 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: CHR51_RS12040
• Protein ID: WP_003405863.1
• Coordinates: 2453314..2453511 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHR51_RS12005	2449293..2450063	+	771	Protein_2274	adenylate/guanylate cyclase domain-containing protein	-
CHR51_RS12010	2450096..2450956	+	861	WP_003898734.1	hypothetical protein	-
CHR51_RS12015	2450971..2451294	-	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
CHR51_RS12020	2451537..2451812	+	276	WP_003405867.1	hypothetical protein	-
CHR51_RS12025	2451739..2451924	-	186	WP_003901093.1	hypothetical protein	-
CHR51_RS12030	2452042..2452740	-	699	WP_003898733.1	hypothetical protein	-
CHR51_RS12035	2452943..2453317	-	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
CHR51_RS12040	2453314..2453511	-	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CHR51_RS12045	2453599..2454672	-	1074	WP_003898732.1	redox-regulated ATPase YchF	-
CHR51_RS12050	2454735..2455718	+	984	WP_003898731.1	hypothetical protein	-
CHR51_RS12055	2455735..2456742	-	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
CHR51_RS12060	2456832..2457470	+	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T79287 WP_003405865.1 NZ_CP022577:c2453317-2452943 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T79287 NZ_CP022577:c2453317-2452943 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT79287 WP_003405863.1 NZ_CP022577:c2453511-2453314 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT79287 NZ_CP022577:c2453511-2453314 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07