Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2001392..2002008 Replicon chromosome
Accession NZ_CP022577
Organism Mycobacterium tuberculosis strain WC078

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag CHR51_RS09905 Protein ID WP_003407593.1
Coordinates 2001392..2001709 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag CHR51_RS09910 Protein ID WP_003900349.1
Coordinates 2001706..2002008 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHR51_RS09875 1996401..1997429 - 1029 WP_003407612.1 glycosyltransferase family 2 protein -
CHR51_RS21800 1997699..1997923 + 225 WP_160522526.1 NUDIX domain-containing protein -
CHR51_RS09885 1997933..1998145 + 213 WP_003898900.1 dodecin family protein -
CHR51_RS09890 1998341..1998976 + 636 WP_071854212.1 class I SAM-dependent methyltransferase -
CHR51_RS09895 1999049..2000338 - 1290 WP_003902090.1 serine hydrolase -
CHR51_RS09900 2000391..2001395 - 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
CHR51_RS09905 2001392..2001709 - 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
CHR51_RS09910 2001706..2002008 - 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
CHR51_RS09915 2002022..2004274 - 2253 WP_003407587.1 methylmalonyl-CoA mutase -
CHR51_RS09920 2004275..2006122 - 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T79283 WP_003407593.1 NZ_CP022577:c2001709-2001392 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T79283 NZ_CP022577:c2001709-2001392 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT79283 WP_003900349.1 NZ_CP022577:c2002008-2001706 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT79283 NZ_CP022577:c2002008-2001706 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References