Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 826989..827620 Replicon chromosome
Accession NZ_CP022577
Organism Mycobacterium tuberculosis strain WC078

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag CHR51_RS04190 Protein ID WP_003413174.1
Coordinates 826989..827366 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag CHR51_RS04195 Protein ID WP_003413167.1
Coordinates 827363..827620 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHR51_RS04150 822456..822968 + 513 WP_003413197.1 Holliday junction resolvase RuvX -
CHR51_RS04155 822961..824214 + 1254 WP_003413196.1 endolytic transglycosylase MltG -
CHR51_RS04160 824211..825020 + 810 WP_003413193.1 shikimate dehydrogenase -
CHR51_RS04165 825032..825451 + 420 WP_003413190.1 A24 family peptidase -
CHR51_RS04175 825862..826107 + 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
CHR51_RS04180 826104..826499 + 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
CHR51_RS04185 826599..826973 + 375 WP_003413177.1 hypothetical protein -
CHR51_RS04190 826989..827366 - 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
CHR51_RS04195 827363..827620 - 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
CHR51_RS04200 827659..828072 - 414 WP_003413164.1 PIN domain nuclease -
CHR51_RS04205 828165..828442 - 278 Protein_797 type II toxin-antitoxin system VapB family antitoxin -
CHR51_RS04210 828439..829101 - 663 WP_003905878.1 hypothetical protein -
CHR51_RS04215 829098..829757 - 660 WP_003902296.1 hypothetical protein -
CHR51_RS04225 829884..831095 - 1212 WP_003900845.1 alpha/beta hydrolase family protein -
CHR51_RS04230 831391..831798 - 408 WP_003413029.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T79265 WP_003413174.1 NZ_CP022577:c827366-826989 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T79265 NZ_CP022577:c827366-826989 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT79265 WP_003413167.1 NZ_CP022577:c827620-827363 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT79265 NZ_CP022577:c827620-827363 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References