Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 585249..585819 Replicon chromosome
Accession NZ_CP022577
Organism Mycobacterium tuberculosis strain WC078

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag CHR51_RS02820 Protein ID WP_003414166.1
Coordinates 585463..585819 (+) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag CHR51_RS02815 Protein ID WP_003901465.1
Coordinates 585249..585479 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHR51_RS02775 580267..580578 - 312 WP_003414190.1 hypothetical protein -
CHR51_RS02780 580683..580940 - 258 WP_003899489.1 hypothetical protein -
CHR51_RS02785 581174..582328 - 1155 WP_003899488.1 DDE-type integrase/transposase/recombinase -
CHR51_RS02790 582527..582718 - 192 WP_003414184.1 hypothetical protein -
CHR51_RS02795 582715..583119 - 405 WP_003414181.1 hypothetical protein -
CHR51_RS21655 583267..583521 + 255 WP_003917684.1 hypothetical protein -
CHR51_RS02805 583697..583972 - 276 WP_003911993.1 DUF1778 domain-containing protein -
CHR51_RS02810 584163..585206 + 1044 WP_003414172.1 DUF2293 domain-containing protein -
CHR51_RS02815 585249..585479 + 231 WP_003901465.1 antitoxin MazE Antitoxin
CHR51_RS02820 585463..585819 + 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
CHR51_RS02825 585921..587570 - 1650 WP_003899485.1 CocE/NonD family hydrolase -
CHR51_RS02830 587589..588218 - 630 WP_003414158.1 DUF3558 family protein -
CHR51_RS02835 588349..588675 + 327 WP_003414157.1 hypothetical protein -
CHR51_RS02840 588679..590367 + 1689 WP_003414155.1 alpha/beta hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 574361..614723 40362


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T79259 WP_003414166.1 NZ_CP022577:585463-585819 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T79259 NZ_CP022577:585463-585819 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT79259 WP_003901465.1 NZ_CP022577:585249-585479 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT79259 NZ_CP022577:585249-585479 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References