Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 143933..144669 Replicon plasmid pBIC-1a
Accession NZ_CP022574
Organism Klebsiella pneumoniae strain BIC-1

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CHX41_RS28180 Protein ID WP_003026803.1
Coordinates 144187..144669 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CHX41_RS28175 Protein ID WP_003026799.1
Coordinates 143933..144199 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHX41_RS28130 139995..140357 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
CHX41_RS28135 140407..140757 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
CHX41_RS28140 141115..141384 + 270 WP_004152102.1 hypothetical protein -
CHX41_RS28145 141372..141947 + 576 WP_004152103.1 hypothetical protein -
CHX41_RS28150 141978..142472 + 495 WP_004152104.1 DNA-binding protein -
CHX41_RS28155 142516..142884 + 369 WP_004152105.1 hypothetical protein -
CHX41_RS28160 142918..143121 + 204 WP_004152106.1 hemolysin expression modulator Hha -
CHX41_RS28165 143170..143427 + 258 WP_004152107.1 hypothetical protein -
CHX41_RS28170 143503..143757 + 255 WP_004152108.1 hypothetical protein -
CHX41_RS28175 143933..144199 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CHX41_RS28180 144187..144669 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CHX41_RS28185 144881..146227 + 1347 WP_020314316.1 ISNCY family transposase -
CHX41_RS28190 146728..147982 - 1255 Protein_155 IS3 family transposase -
CHX41_RS28195 148070..149032 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaOXA-9 / blaTEM-1A / aph(3')-Ia - 1..170415 170415


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T79253 WP_003026803.1 NZ_CP022574:144187-144669 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T79253 NZ_CP022574:144187-144669 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT79253 WP_003026799.1 NZ_CP022574:143933-144199 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT79253 NZ_CP022574:143933-144199 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References