Detailed information of TA system TA079232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 273999..274579
• Replicon: plasmid pSK04
• Accession: NZ_CP022568
• Organism: Rhizobium leguminosarum bv. viciae strain BIHB 1148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CHY08_RS32730
• Protein ID: WP_094088894.1
• Coordinates: 274196..274579 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0T6ZM79
• Locus tag: CHY08_RS32725
• Protein ID: WP_017958375.1
• Coordinates: 273999..274199 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHY08_RS32700	269400..270233	+	834	WP_094088892.1	AraC family transcriptional regulator	-
CHY08_RS32705	270585..271427	-	843	WP_017958379.1	type 1 glutamine amidotransferase domain-containing protein	-
CHY08_RS32710	271483..271785	-	303	WP_017958378.1	antibiotic biosynthesis monooxygenase	-
CHY08_RS32715	271799..272590	-	792	WP_094088893.1	SDR family oxidoreductase	-
CHY08_RS32720	272688..273614	+	927	WP_017958376.1	LysR family transcriptional regulator	-
CHY08_RS32725	273999..274199	+	201	WP_017958375.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CHY08_RS32730	274196..274579	+	384	WP_094088894.1	type II toxin-antitoxin system VapC family toxin	Toxin
CHY08_RS32735	274833..276230	-	1398	WP_017958373.1	dihydrolipoyl dehydrogenase	-
CHY08_RS32740	276236..277474	-	1239	WP_017958372.1	2-oxo acid dehydrogenase subunit E2	-
CHY08_RS32745	277478..278491	-	1014	WP_017958371.1	alpha-ketoacid dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..326874	326874

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14075.15 Da
• Isoelectric Point: 6.9169

>T79232 WP_094088894.1 NZ_CP022568:274196-274579 [Rhizobium leguminosarum bv. viciae]
VILADTSIWIDHFRHLDAELRTIIEDDRLLCHPAVAGELALGSLRDRGRVIAFLAAQRQAFVATHDEVMTMIDRHGIFSM
GIGYTDAHLLASIMLDQRAALWTRDKRLQAAAEKAGASLHKPANAHN

Nucleotide

• Length: 384 bp

>T79232 NZ_CP022568:274196-274579 [Rhizobium leguminosarum bv. viciae]
GTGATACTGGCAGACACTTCTATATGGATCGATCATTTCCGCCATCTTGATGCAGAGCTTCGGACGATCATTGAGGACGA
TCGTCTACTCTGCCATCCGGCTGTGGCTGGCGAACTGGCGCTTGGCAGCCTTCGAGATCGCGGGCGCGTGATAGCGTTTC
TGGCGGCCCAGCGCCAAGCGTTCGTCGCAACGCACGACGAAGTGATGACAATGATTGATCGTCATGGCATTTTCAGTATG
GGCATCGGCTATACAGATGCCCACTTGCTCGCCTCGATAATGCTTGATCAGCGAGCGGCGTTATGGACCAGGGACAAGCG
TCTGCAGGCGGCGGCCGAAAAGGCAGGCGCTTCATTGCATAAGCCTGCGAATGCTCACAACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7124.23 Da
• Isoelectric Point: 11.3760

>AT79232 WP_017958375.1 NZ_CP022568:273999-274199 [Rhizobium leguminosarum bv. viciae]
MRSTINLDDTLMERARSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT79232 NZ_CP022568:273999-274199 [Rhizobium leguminosarum bv. viciae]
ATGCGATCGACTATCAATCTCGACGACACTCTCATGGAAAGGGCCAGGTCCCTGACCGGCACAAAAGAAACCGCTGCTCT
AGTCCGCCAGGCGTTGGAGACGCTCGTCCGAGTAGAGTCGGGAAAACGCCTCATCGCGCTCGGCGGAACTATGCCCGATG
CAGAGGCAGCTCCACGTCGCCGAAGCGCAGCTGCCAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0T6ZM79