Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relB-parE/ParE-RHH
Location 84074..84630 Replicon plasmid pSK04
Accession NZ_CP022568
Organism Rhizobium leguminosarum bv. viciae strain BIHB 1148

Toxin (Protein)


Gene name parE Uniprot ID A0A198YJZ4
Locus tag CHY08_RS31790 Protein ID WP_017958251.1
Coordinates 84340..84630 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A7Z0UH60
Locus tag CHY08_RS31785 Protein ID WP_017958252.1
Coordinates 84074..84352 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHY08_RS31765 79347..80234 - 888 WP_017958256.1 sugar ABC transporter permease -
CHY08_RS31770 80331..81563 - 1233 WP_017958255.1 ABC transporter substrate-binding protein -
CHY08_RS31775 81622..82575 - 954 WP_094088848.1 sugar phosphate isomerase/epimerase -
CHY08_RS31780 82796..83866 + 1071 WP_017958253.1 LacI family transcriptional regulator -
CHY08_RS31785 84074..84352 + 279 WP_017958252.1 CopG family ribbon-helix-helix protein Antitoxin
CHY08_RS31790 84340..84630 + 291 WP_017958251.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CHY08_RS31795 84794..85237 + 444 WP_017958250.1 acetyl-CoA carboxylase biotin carboxyl carrier protein subunit -
CHY08_RS31800 85237..86622 + 1386 WP_017958249.1 acetyl-CoA carboxylase biotin carboxylase subunit -
CHY08_RS31805 86619..87056 + 438 WP_017958248.1 biotin carboxyl carrier protein -
CHY08_RS31810 87053..87760 + 708 WP_094088849.1 5-oxoprolinase subunit PxpB -
CHY08_RS31815 87757..88752 + 996 WP_017958246.1 biotin-dependent carboxyltransferase family protein -
CHY08_RS31820 88790..89557 + 768 WP_017958245.1 LamB/YcsF family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..326874 326874


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-94)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-42)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11035.72 Da        Isoelectric Point: 8.5860

>T79231 WP_017958251.1 NZ_CP022568:84340-84630 [Rhizobium leguminosarum bv. viciae]
MPQVVFSPAAIRDLERLREFLRLKNPSAAKRAAETILNGLRVLSAHPHIGRLIEDLPEHYREWLIDFGDSGYIARYRIDG
DILTILTVRHQKEAGF

Download         Length: 291 bp

>T79231 NZ_CP022568:84340-84630 [Rhizobium leguminosarum bv. viciae]
ATGCCACAAGTAGTCTTCTCCCCCGCCGCAATCCGCGACCTTGAGCGGCTACGGGAGTTCTTGCGGCTGAAAAACCCATC
GGCCGCAAAGCGAGCAGCCGAAACTATTCTCAACGGCTTGAGAGTCCTCAGCGCACATCCCCATATTGGACGCCTCATCG
AGGACTTGCCCGAGCACTATCGGGAGTGGCTCATCGATTTTGGAGATAGCGGATACATCGCCCGTTATCGTATTGATGGC
GATATTTTGACGATCCTAACCGTACGGCATCAGAAGGAAGCAGGGTTCTGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10652.90 Da        Isoelectric Point: 5.2246

>AT79231 WP_017958252.1 NZ_CP022568:84074-84352 [Rhizobium leguminosarum bv. viciae]
MASPTSLKLDDELKGRVQQLAEARRRSSHWIMREAIAQYVEREEKREALRQETLDAWNEFKATGLHVTGAEVEKWLSTWG
TDDELSAPECHK

Download         Length: 279 bp

>AT79231 NZ_CP022568:84074-84352 [Rhizobium leguminosarum bv. viciae]
ATGGCATCGCCAACGTCGCTCAAGCTCGATGATGAGCTAAAGGGCCGCGTTCAGCAACTGGCCGAAGCTCGCCGCCGCTC
GTCTCACTGGATCATGCGTGAAGCTATTGCGCAATATGTTGAGCGCGAGGAGAAGCGTGAGGCGCTGCGACAGGAGACGC
TTGACGCCTGGAATGAATTTAAAGCGACGGGACTGCATGTCACCGGCGCCGAGGTCGAAAAATGGCTTTCGACTTGGGGA
ACTGACGACGAGTTGTCCGCACCCGAATGCCACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A198YJZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z0UH60

References