Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 636339..637030 Replicon plasmid pSK01
Accession NZ_CP022565
Organism Rhizobium leguminosarum bv. viciae strain BIHB 1148

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag CHY08_RS25165 Protein ID WP_094088148.1
Coordinates 636602..637030 (+) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag CHY08_RS25160 Protein ID WP_094088147.1
Coordinates 636339..636605 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHY08_RS25125 631643..631900 + 258 WP_003550229.1 hypothetical protein -
CHY08_RS25130 631900..632271 + 372 WP_017956568.1 type II toxin-antitoxin system RelE/ParE family toxin -
CHY08_RS25135 632432..633157 - 726 WP_017956569.1 amino acid ABC transporter ATP-binding protein -
CHY08_RS25140 633135..633794 - 660 WP_017956570.1 amino acid ABC transporter permease -
CHY08_RS25145 633791..634462 - 672 WP_094088145.1 amino acid ABC transporter permease -
CHY08_RS25150 634542..635327 - 786 WP_094088146.1 transporter substrate-binding domain-containing protein -
CHY08_RS25155 635321..636082 - 762 WP_131627366.1 GntR family transcriptional regulator -
CHY08_RS25160 636339..636605 + 267 WP_094088147.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CHY08_RS25165 636602..637030 + 429 WP_094088148.1 type II toxin-antitoxin system VapC family toxin Toxin
CHY08_RS25170 637071..637841 - 771 WP_017956575.1 amino acid ABC transporter ATP-binding protein -
CHY08_RS25175 637854..638513 - 660 WP_024319797.1 amino acid ABC transporter permease -
CHY08_RS25180 638518..639180 - 663 WP_094088149.1 amino acid ABC transporter permease -
CHY08_RS25185 639294..640133 - 840 WP_017956578.1 transporter substrate-binding domain-containing protein -
CHY08_RS25190 640297..640512 - 216 Protein_611 PAS domain S-box protein -
CHY08_RS25195 640499..640827 - 329 Protein_612 flagellar protein -
CHY08_RS25200 640833..641480 - 648 WP_017956580.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..976232 976232


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(9-72)


Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15890.20 Da        Isoelectric Point: 4.6791

>T79229 WP_094088148.1 NZ_CP022565:636602-637030 [Rhizobium leguminosarum bv. viciae]
VRLLLDTNVLSEVTRPRPDARVLEWLDSLDEDRSFISVVSIAEIRRGVALMESGRKRDALAEWLAQDLPQRFEQRVTPVD
QPVAIVWGDLMGLAKRSGRGLSSMDGLIAATAIAHDLTLATRNIKDFEGFGIELVDPWIERP

Download         Length: 429 bp

>T79229 NZ_CP022565:636602-637030 [Rhizobium leguminosarum bv. viciae]
GTGAGACTTCTGCTGGACACCAATGTGCTTTCCGAGGTGACAAGGCCGCGTCCCGATGCACGTGTCCTAGAATGGCTGGA
CAGTCTCGACGAGGATCGCTCATTCATCAGCGTCGTATCGATCGCGGAGATCCGGCGTGGTGTTGCATTGATGGAAAGCG
GCCGGAAGCGCGACGCGCTGGCTGAATGGCTTGCACAGGACCTGCCGCAGCGCTTCGAGCAGCGCGTCACCCCTGTCGAC
CAGCCGGTTGCGATAGTCTGGGGCGACCTGATGGGGCTCGCAAAGCGAAGCGGACGCGGCTTGTCGTCAATGGACGGCTT
GATTGCCGCCACCGCGATTGCTCACGATCTCACTCTCGCCACCCGTAATATAAAGGATTTCGAAGGTTTTGGGATCGAGC
TTGTCGATCCTTGGATAGAGCGCCCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9918.26 Da        Isoelectric Point: 8.7461

>AT79229 WP_094088147.1 NZ_CP022565:636339-636605 [Rhizobium leguminosarum bv. viciae]
MQPSRPEDKNWTVASAKAKLSEVIERAQATPQTIMRNGKPSVVVVSAEEWQRKTARKGTLAEFLMQSPLSGADLDLERQR
DEPRDLQL

Download         Length: 267 bp

>AT79229 NZ_CP022565:636339-636605 [Rhizobium leguminosarum bv. viciae]
ATGCAGCCATCACGACCAGAGGATAAAAATTGGACCGTCGCCAGTGCGAAGGCCAAGCTGTCTGAGGTTATCGAACGCGC
GCAAGCAACGCCGCAAACGATCATGCGCAATGGTAAACCAAGCGTCGTCGTCGTTTCGGCCGAAGAATGGCAGCGGAAGA
CCGCGCGCAAGGGCACCCTTGCAGAGTTTCTGATGCAATCGCCGCTGAGTGGCGCCGATCTGGACCTCGAACGACAGCGC
GACGAACCGCGTGATCTCCAGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References