Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 435435..436111 Replicon plasmid pSK01
Accession NZ_CP022565
Organism Rhizobium leguminosarum bv. viciae strain BIHB 1148

Toxin (Protein)


Gene name vapC Uniprot ID A0A6N9Z943
Locus tag CHY08_RS24215 Protein ID WP_017957377.1
Coordinates 435686..436111 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7V8HB42
Locus tag CHY08_RS24210 Protein ID WP_017957376.1
Coordinates 435435..435689 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHY08_RS24195 431004..432602 - 1599 WP_094088035.1 ABC transporter substrate-binding protein -
CHY08_RS24200 432586..433329 - 744 WP_094088036.1 FadR family transcriptional regulator -
CHY08_RS24205 433500..434716 - 1217 Protein_416 GGDEF domain-containing protein -
CHY08_RS24210 435435..435689 + 255 WP_017957376.1 plasmid stabilization protein Antitoxin
CHY08_RS24215 435686..436111 + 426 WP_017957377.1 type II toxin-antitoxin system VapC family toxin Toxin
CHY08_RS24220 436410..438578 + 2169 WP_094088037.1 TonB-dependent siderophore receptor -
CHY08_RS24225 438588..439370 + 783 WP_094088038.1 siderophore-iron reductase FhuF -
CHY08_RS24230 439453..439944 - 492 WP_094088039.1 GNAT family N-acetyltransferase -
CHY08_RS24235 440108..440767 + 660 WP_017957381.1 sigma-70 family RNA polymerase sigma factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..976232 976232


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15262.51 Da        Isoelectric Point: 4.8011

>T79228 WP_017957377.1 NZ_CP022565:435686-436111 [Rhizobium leguminosarum bv. viciae]
MILIDTNVISEPWKPTPDNQVLAWIDAQAVETLFVSAITVAELRFGIAAMPSGRRKTVLYDRLEQEVLPLFSGRVLSFDM
AASQSYSELMARARVAGRAIGKADAYIAATAAANGFIVATRDTSPFEAAGLNVINPWYAPT

Download         Length: 426 bp

>T79228 NZ_CP022565:435686-436111 [Rhizobium leguminosarum bv. viciae]
ATGATCCTGATCGATACCAACGTCATCTCGGAGCCATGGAAGCCCACACCCGATAATCAGGTACTGGCGTGGATCGATGC
CCAAGCAGTGGAAACGCTGTTCGTGTCGGCGATTACAGTAGCTGAGTTACGGTTCGGCATTGCCGCGATGCCTAGCGGAA
GACGAAAGACCGTCCTCTACGATCGACTAGAACAGGAAGTTCTGCCTCTATTTTCAGGGCGCGTTCTATCCTTCGACATG
GCTGCGTCACAGAGCTATTCGGAATTGATGGCGCGAGCACGCGTAGCAGGTCGGGCAATCGGTAAAGCAGATGCCTATAT
CGCCGCAACTGCCGCGGCGAACGGATTTATTGTCGCCACCCGCGACACTAGTCCCTTCGAAGCCGCAGGACTTAACGTTA
TCAACCCGTGGTACGCACCAACATAG

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9218.42 Da        Isoelectric Point: 6.2221

>AT79228 WP_017957376.1 NZ_CP022565:435435-435689 [Rhizobium leguminosarum bv. viciae]
MAAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDILETAVRPAERVMLGSLLASIGREAGLSNNDVDNLQLVRDKAPAEP
MSFE

Download         Length: 255 bp

>AT79228 NZ_CP022565:435435-435689 [Rhizobium leguminosarum bv. viciae]
ATGGCTGCAGTTACAATACGCAATTTGTCTGACGAGACACATCGTGCCTTAAGAGTGAGGGCAGCTCATCACGGCCGCAG
TACAGAAGCAGAAATCCGTGACATCCTTGAAACGGCGGTACGGCCAGCTGAGCGCGTCATGTTGGGCTCGTTGCTAGCAT
CGATCGGGCGCGAGGCAGGGCTTTCCAACAACGACGTGGATAATCTGCAGCTCGTCCGAGACAAAGCGCCTGCGGAACCT
ATGAGTTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6N9Z943

Antitoxin

Source ID Structure
AlphaFold DB A0A7V8HB42

References