Detailed information of TA system TA079227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 159703..160283
• Replicon: plasmid pSK01
• Accession: NZ_CP022565
• Organism: Rhizobium leguminosarum bv. viciae strain BIHB 1148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1B1CJ75
• Locus tag: CHY08_RS22905
• Protein ID: WP_033182978.1
• Coordinates: 159900..160283 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CHY08_RS22900
• Protein ID: WP_094087847.1
• Coordinates: 159703..159903 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHY08_RS35020	156086..156154	+	69	Protein_151	terminase small subunit	-
CHY08_RS22880	156329..156688	+	360	WP_094087844.1	hypothetical protein	-
CHY08_RS34545	157038..157192	-	155	Protein_153	transposase domain-containing protein	-
CHY08_RS22890	158192..158560	-	369	WP_094087845.1	response regulator	-
CHY08_RS22895	158560..159483	-	924	WP_172426341.1	PAS domain-containing protein	-
CHY08_RS22900	159703..159903	+	201	WP_094087847.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CHY08_RS22905	159900..160283	+	384	WP_033182978.1	type II toxin-antitoxin system VapC family toxin	Toxin
CHY08_RS22910	160636..161178	-	543	WP_094087848.1	GNAT family N-acetyltransferase	-
CHY08_RS22915	161178..161486	-	309	WP_003563077.1	DUF1778 domain-containing protein	-
CHY08_RS22920	162080..162459	+	380	Protein_160	hypothetical protein	-
CHY08_RS22925	162521..163015	-	495	WP_094087849.1	lipocalin-like domain-containing protein	-
CHY08_RS22930	163069..163901	-	833	Protein_162	SDR family oxidoreductase	-
CHY08_RS22935	163993..164892	+	900	WP_094087850.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..976232	976232

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14147.33 Da
• Isoelectric Point: 9.1808

>T79227 WP_033182978.1 NZ_CP022565:159900-160283 [Rhizobium leguminosarum bv. viciae]
VILADTSIWIDHFRHVDAELRTIIEDDRLLCHPAVVGELALGSLRDRGRVITFLAAQRQAFVATHDEVMTMIDRHGIFSM
GIGYTDAHLLASILLDRRAALWTRDKRLQAAAKKAGASLHKPANARN

Nucleotide

• Length: 384 bp

>T79227 NZ_CP022565:159900-160283 [Rhizobium leguminosarum bv. viciae]
GTGATACTGGCAGACACTTCTATATGGATCGATCATTTCCGCCATGTTGATGCGGAACTTCGGACGATCATTGAGGACGA
TCGTCTACTCTGCCATCCGGCTGTGGTTGGCGAACTGGCGCTTGGCAGCCTTCGAGATCGCGGGCGCGTGATAACGTTTC
TGGCAGCCCAGCGCCAAGCGTTCGTCGCAACGCACGACGAAGTGATGACAATGATTGATCGTCACGGCATTTTCAGTATG
GGCATCGGCTATACAGATGCCCACTTGCTCGCCTCGATACTGCTTGATCGGCGCGCGGCTTTATGGACCAGGGACAAGCG
TCTTCAGGCGGCGGCCAAAAAGGCAGGCGCTTCATTGCATAAGCCGGCCAATGCTCGCAACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7114.25 Da
• Isoelectric Point: 11.1107

>AT79227 WP_094087847.1 NZ_CP022565:159703-159903 [Rhizobium leguminosarum bv. viciae]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLMALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT79227 NZ_CP022565:159703-159903 [Rhizobium leguminosarum bv. viciae]
ATGCGATCGACTATCAATCTAGACGACACTCTCATGGAAAGGGCCAAGTCCCTGACCGGCACAAAAGAAACCGCTGCTCT
AGTCCGCCAGGCGTTGGAGACGCTTGTCCGAGTAGAGTCCGGAAAACGCCTCATGGCGCTCGGGGGAACTATGCCCGATG
CAGAGGCAGCTCCACGTCGCCGGAGCGCAGCTGCCAAGTGA

Structures

Toxin

• AlphaFold DB: A0A1B1CJ75