Detailed information of TA system TA079222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1670572..1671614
• Replicon: chromosome
• Accession: NZ_CP022562
• Organism: Pseudomonas monteilii strain B5

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CHR29_RS07785
• Protein ID: WP_003153636.1
• Coordinates: 1671039..1671614 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: CHR29_RS07780
• Protein ID: WP_003050245.1
• Coordinates: 1670572..1671042 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHR29_RS07745	1665951..1667369	-	1419	WP_004350605.1	TIGR03752 family integrating conjugative element protein	-
CHR29_RS07750	1667359..1668270	-	912	WP_004350604.1	TIGR03749 family integrating conjugative element protein	-
CHR29_RS07755	1668267..1668959	-	693	WP_012614071.1	TIGR03746 family integrating conjugative element protein	-
CHR29_RS07760	1668956..1669366	-	411	WP_003821108.1	TIGR03750 family conjugal transfer protein	-
CHR29_RS07765	1669379..1669738	-	360	WP_003153634.1	TIGR03745 family integrating conjugative element membrane protein	-
CHR29_RS07770	1669755..1669988	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
CHR29_RS07775	1669985..1670368	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
CHR29_RS07780	1670572..1671042	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
CHR29_RS07785	1671039..1671614	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
CHR29_RS07790	1671632..1672546	+	915	WP_003050256.1	AAA family ATPase	-
CHR29_RS07795	1672543..1673013	+	471	WP_003153638.1	hypothetical protein	-
CHR29_RS07800	1673010..1673510	+	501	WP_003090159.1	hypothetical protein	-
CHR29_RS07805	1673510..1674412	+	903	WP_003153640.1	nucleotidyltransferase domain-containing protein	-
CHR29_RS07810	1674451..1675176	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1607200..1724919	117719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T79222 WP_003153636.1 NZ_CP022562:1671039-1671614 [Pseudomonas monteilii]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T79222 NZ_CP022562:1671039-1671614 [Pseudomonas monteilii]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT79222 WP_003050245.1 NZ_CP022562:1670572-1671042 [Pseudomonas monteilii]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT79222 NZ_CP022562:1670572-1671042 [Pseudomonas monteilii]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68