Detailed information of TA system TA079219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1939987..1941026
• Replicon: chromosome
• Accession: NZ_CP022561
• Organism: Pseudomonas putida strain B4

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CHN49_RS09015
• Protein ID: WP_019750859.1
• Coordinates: 1939987..1940562 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A173HI04
• Locus tag: CHN49_RS09020
• Protein ID: WP_003251469.1
• Coordinates: 1940559..1941026 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHN49_RS09005	1935604..1936839	+	1236	WP_121783129.1	FAD-dependent oxidoreductase	-
CHN49_RS09010	1936843..1939773	-	2931	WP_121783130.1	aminotransferase	-
CHN49_RS09015	1939987..1940562	-	576	WP_019750859.1	PIN domain-containing protein	Toxin
CHN49_RS09020	1940559..1941026	-	468	WP_003251469.1	helix-turn-helix domain-containing protein	Antitoxin
CHN49_RS09025	1941171..1942778	-	1608	WP_121783131.1	ABC transporter ATP-binding protein	-
CHN49_RS09030	1942780..1943799	-	1020	WP_003251467.1	ABC transporter permease	-
CHN49_RS09035	1943801..1944874	-	1074	WP_003251466.1	microcin C ABC transporter permease YejB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21683.92 Da
• Isoelectric Point: 5.5710

>T79219 WP_019750859.1 NZ_CP022561:c1940562-1939987 [Pseudomonas putida]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSASIHDEWKRNLLLNRPDLTPEHLDRTSSLMDAAVPDALVTDY
DSLVEGLDLPDRDDRHVLAAAIKCNASVIVTFNLKDFPKAVLGAFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRISLKN
PPHNVQQYLDRLLQQKLPETVKLLSAFKFLL

Nucleotide

• Length: 576 bp

>T79219 NZ_CP022561:c1940562-1939987 [Pseudomonas putida]
ATGAAGCACTCGCCCTTCACGGCCATATACGACGCGAATGTACTTTACCCTGCCCCACTGCGTGATTTCCTGATGAACCT
AGCGCTGACGGGCATCTACCGGGCACGTTGGTCAGCGAGCATTCATGACGAGTGGAAGCGCAATCTGCTACTCAACCGAC
CTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTAATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTAC
GATTCACTGGTCGAAGGGCTGGACTTGCCAGATCGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTC
GGTCATCGTCACCTTCAATTTGAAGGATTTTCCCAAGGCGGTTCTTGGTGCCTTCGATATTGAACCCTTGCATCCGGACG
ACTTCATCGCTGACCTTTGGGACCTGGACAAGGCAGCAGTCCTCGAAGCTGCCCAGCGGCAGCGAATATCGCTGAAGAAC
CCACCGCACAACGTACAGCAGTATCTCGACAGGCTGCTTCAGCAGAAGCTTCCCGAGACAGTGAAACTGCTTTCAGCATT
CAAGTTCCTCCTCTGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17229.71 Da
• Isoelectric Point: 6.6505

>AT79219 WP_003251469.1 NZ_CP022561:c1941026-1940559 [Pseudomonas putida]
MTTANLSRHVLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPHLVKLLDEAVIPHTKTGRHRRVKFSDLVAYKDKRDGVSRTAMDALAAQAQELKMGYE

Nucleotide

• Length: 468 bp

>AT79219 NZ_CP022561:c1941026-1940559 [Pseudomonas putida]
ATGACCACCGCCAACCTCTCTCGCCATGTGCTTCCTGATGAGAAAGAAATTGCCGTGGCAGTCGAGTCAAGCCGCACGCT
GGCAGCGTTCCTCTCGACAAAATCAGATACCCAACGGATCGAACTGCTCAACGAGGAAAATCAGCGCCAGGTTGTTGAAG
TACCTACTTTCGCCCTACGCCTATTCGGCGAGATTCTCAGCGAACTGGCCCTGGGTAACTCAGTCAAAGTCGTTCCAATC
CACGCCGAACTAACTACCCAGGAAGCAGCAGACCTGCTTAACGTCTCCCGGCCGCACTTGGTAAAGCTGCTCGACGAAGC
GGTGATTCCGCACACCAAGACAGGCCGCCATCGTCGTGTCAAATTCTCCGACCTGGTTGCCTACAAGGACAAACGTGATG
GGGTGAGCCGGACTGCGATGGATGCGCTAGCAGCCCAAGCTCAGGAATTGAAGATGGGGTACGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A173HI04