Detailed information of TA system TA079217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 946404..946990
• Replicon: chromosome
• Accession: NZ_CP022561
• Organism: Pseudomonas putida strain B4

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A0C5RWB6
• Locus tag: CHN49_RS04390
• Protein ID: WP_019472637.1
• Coordinates: 946404..946697 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CHN49_RS04395
• Protein ID: WP_014861463.1
• Coordinates: 946694..946990 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHN49_RS04365	942005..942859	+	855	WP_014861459.1	putative selenate ABC transporter substrate-binding protein	-
CHN49_RS04370	942856..943665	+	810	WP_121782879.1	phosphonate ABC transporter ATP-binding protein	-
CHN49_RS04375	943659..944486	+	828	WP_004576350.1	ABC transporter permease subunit	-
CHN49_RS04380	944483..945250	+	768	WP_121782880.1	phosphonate ABC transporter, permease protein PhnE	-
CHN49_RS04385	945287..946180	-	894	WP_014861462.1	hypothetical protein	-
CHN49_RS04390	946404..946697	+	294	WP_019472637.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CHN49_RS04395	946694..946990	+	297	WP_014861463.1	putative addiction module antidote protein	Antitoxin
CHN49_RS04400	947001..947480	-	480	WP_014861464.1	GNAT family N-acetyltransferase	-
CHN49_RS04405	947589..948236	-	648	WP_121782881.1	serine protease	-
CHN49_RS04410	948580..949002	+	423	WP_012051239.1	VOC family protein	-
CHN49_RS04420	949588..950979	+	1392	WP_205602678.1	FAD-dependent oxidoreductase	-
CHN49_RS04425	951059..951556	+	498	WP_014589747.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-92)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10765.62 Da
• Isoelectric Point: 10.9252

>T79217 WP_019472637.1 NZ_CP022561:946404-946697 [Pseudomonas putida]
MFLVEQTESFAAWLSSLRDLRARLAIGRRLERAAAGNLGDFKWLGGGIGELRIDVAAGYRVYFTQKGQRLVLLLVGGDKS
TQAADILKARKLLKEMK

Nucleotide

• Length: 294 bp

>T79217 NZ_CP022561:946404-946697 [Pseudomonas putida]
ATGTTTCTCGTTGAACAGACGGAAAGCTTTGCCGCTTGGCTATCGAGCTTGAGAGATTTGAGGGCCAGGCTGGCGATAGG
TCGTAGGCTGGAGCGAGCAGCGGCGGGCAATCTTGGTGACTTCAAGTGGCTTGGAGGCGGTATTGGTGAGCTTCGCATCG
ACGTAGCTGCCGGTTATCGCGTCTATTTCACGCAAAAGGGGCAGAGGTTGGTTTTACTGCTGGTCGGCGGCGACAAGTCC
ACCCAAGCCGCCGACATCCTCAAAGCCAGAAAGCTGCTGAAGGAGATGAAATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10706.42 Da
• Isoelectric Point: 6.7249

>AT79217 WP_014861463.1 NZ_CP022561:946694-946990 [Pseudomonas putida]
MSEKLIPFDMAALLSSDEAISEYLSQVLSNGDTEEIIRALGHIARARGMTHIAMESGLGRESLYKALSPGAKPRFDTVLR
VIRALGVDLLVQPHAMTR

Nucleotide

• Length: 297 bp

>AT79217 NZ_CP022561:946694-946990 [Pseudomonas putida]
ATGAGCGAAAAATTGATCCCGTTTGATATGGCAGCCTTGTTGAGCAGCGATGAGGCAATCAGTGAATACCTTAGTCAGGT
GCTATCAAATGGCGATACCGAGGAAATCATTCGAGCACTTGGCCATATCGCTCGTGCACGGGGCATGACACATATTGCTA
TGGAGAGTGGGCTGGGTAGAGAAAGCCTGTACAAGGCGCTGAGCCCTGGCGCGAAGCCTCGTTTTGACACTGTATTGAGG
GTCATTCGTGCTCTGGGGGTGGACCTTCTCGTTCAGCCACATGCAATGACCCGCTGA

Structures

Toxin

• AlphaFold DB: A0A0C5RWB6