Detailed information of TA system TA079214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 3418125..3418713
• Replicon: chromosome
• Accession: NZ_CP022560
• Organism: Pseudomonas putida strain B1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: R9VGK1
• Locus tag: CHR26_RS16315
• Protein ID: WP_016488542.1
• Coordinates: 3418411..3418713 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CHR26_RS16310
• Protein ID: WP_016488543.1
• Coordinates: 3418125..3418418 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHR26_RS16290	3413831..3414766	+	936	WP_145913836.1	AEC family transporter	-
CHR26_RS16295	3414875..3416176	-	1302	WP_145913837.1	mechanosensitive ion channel family protein	-
CHR26_RS16300	3416280..3417641	+	1362	WP_023047768.1	DEAD/DEAH box helicase	-
CHR26_RS16305	3417763..3418035	-	273	WP_060519159.1	hypothetical protein	-
CHR26_RS16310	3418125..3418418	-	294	WP_016488543.1	putative addiction module antidote protein	Antitoxin
CHR26_RS16315	3418411..3418713	-	303	WP_016488542.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CHR26_RS16320	3418889..3419284	+	396	WP_063427289.1	hypothetical protein	-
CHR26_RS16325	3419391..3420317	+	927	WP_016488540.1	DMT family transporter	-
CHR26_RS16330	3420365..3421534	-	1170	WP_096426616.1	MFS transporter	-
CHR26_RS16335	3421641..3422534	+	894	WP_145913838.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11379.12 Da
• Isoelectric Point: 10.8638

>T79214 WP_016488542.1 NZ_CP022560:c3418713-3418411 [Pseudomonas putida]
MKKIESSSFRHWVTGLRDLSARARIISRINRLMEGLPGDVSPVGHGVSELKIHYGPGYRVYFHQTGSTFVILLCGGDKSS
QKRDIKVAHQILRSWRMQND

Nucleotide

• Length: 303 bp

>T79214 NZ_CP022560:c3418713-3418411 [Pseudomonas putida]
ATGAAGAAAATCGAATCCAGCAGTTTCAGGCATTGGGTGACCGGACTACGGGACTTAAGCGCCAGAGCCCGAATCATTTC
TCGGATCAACCGGTTGATGGAGGGTCTGCCGGGCGACGTATCTCCGGTAGGCCATGGCGTGAGTGAGCTCAAAATTCACT
ATGGTCCCGGCTATCGGGTGTACTTCCATCAGACGGGCAGCACCTTCGTCATTCTGCTCTGTGGCGGCGACAAAAGCAGT
CAAAAGCGAGACATCAAGGTTGCCCACCAGATTCTGCGTTCGTGGAGGATGCAAAATGACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10446.88 Da
• Isoelectric Point: 5.0637

>AT79214 WP_016488543.1 NZ_CP022560:c3418418-3418125 [Pseudomonas putida]
MTETFSEFDPAAYLTTPEAIAQFMTDALETGDASYVAKAVGVVARAKGMSELAKETGLSREQLYRSFSEHGNPTLKSFLT
VMKALGIDMTARPHLAG

Nucleotide

• Length: 294 bp

>AT79214 NZ_CP022560:c3418418-3418125 [Pseudomonas putida]
ATGACTGAAACCTTCTCTGAATTCGATCCTGCCGCGTACCTCACTACACCTGAAGCAATCGCGCAGTTCATGACCGATGC
ACTTGAAACCGGCGATGCCAGCTATGTCGCTAAAGCTGTCGGCGTGGTTGCACGTGCCAAGGGCATGAGCGAACTGGCGA
AAGAGACGGGGCTGTCCCGAGAGCAGCTTTACAGGTCATTCAGCGAGCATGGCAACCCCACGCTGAAGTCGTTTCTGACC
GTGATGAAGGCATTGGGAATAGACATGACGGCGCGGCCTCACCTCGCTGGCTGA

Structures

Toxin

• AlphaFold DB: R9VGK1