Detailed information of TA system TA079213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3062802..3063318
• Replicon: chromosome
• Accession: NZ_CP022560
• Organism: Pseudomonas putida strain B1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A8I0ZDP4
• Locus tag: CHR26_RS14400
• Protein ID: WP_023047836.1
• Coordinates: 3063031..3063318 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CHR26_RS14395
• Protein ID: WP_145913764.1
• Coordinates: 3062802..3063041 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHR26_RS14375	3057856..3058827	-	972	WP_016488691.1	TRAP transporter substrate-binding protein	-
CHR26_RS14380	3058892..3059773	-	882	WP_016488690.1	SMP-30/gluconolactonase/LRE family protein	-
CHR26_RS14385	3059799..3060605	-	807	WP_023047838.1	NAD(P)-dependent oxidoreductase	-
CHR26_RS14390	3060845..3062082	-	1238	Protein_2743	OprD family porin	-
CHR26_RS14395	3062802..3063041	+	240	WP_145913764.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CHR26_RS14400	3063031..3063318	+	288	WP_023047836.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CHR26_RS14405	3063618..3064154	-	537	WP_145913765.1	Bro-N domain-containing protein	-
CHR26_RS14410	3064391..3065032	+	642	WP_145913766.1	FRG domain-containing protein	-
CHR26_RS14415	3065335..3066585	-	1251	WP_016488688.1	ribonucleotide-diphosphate reductase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11242.07 Da
• Isoelectric Point: 10.1560

>T79213 WP_023047836.1 NZ_CP022560:3063031-3063318 [Pseudomonas putida]
MTYSLDFDARALKEWKKLGDTVRQQFKKKLAEVLLNPRIEANRLHSLPDCYKIKLRSSGFRLVYQVIDQEVVVFVVAVDR
RERDQAYKKAAERLE

Nucleotide

• Length: 288 bp

>T79213 NZ_CP022560:3063031-3063318 [Pseudomonas putida]
ATGACCTATAGCCTCGACTTCGATGCCCGCGCATTGAAAGAGTGGAAGAAGCTGGGCGATACCGTGCGTCAGCAGTTCAA
GAAGAAACTGGCAGAGGTCCTGCTGAACCCTCGAATCGAGGCAAACCGCCTTCACTCGCTGCCGGATTGCTACAAGATAA
AGCTTCGCAGCAGCGGGTTTAGGCTGGTCTATCAGGTCATTGACCAAGAGGTCGTGGTTTTTGTCGTCGCTGTCGATCGC
CGTGAAAGAGACCAAGCTTACAAAAAGGCTGCTGAGCGACTTGAGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9085.47 Da
• Isoelectric Point: 4.8411

>AT79213 WP_145913764.1 NZ_CP022560:3062802-3063041 [Pseudomonas putida]
MASINIRVDDDLKARAYKELERLGVTPSELMRQALQYVAERGKLPFRPVLMTEEDEDLIATVQERLASAKRVRVQLDDL

Nucleotide

• Length: 240 bp

>AT79213 NZ_CP022560:3062802-3063041 [Pseudomonas putida]
ATGGCTTCCATCAACATCCGCGTCGACGATGATCTCAAAGCCCGCGCCTACAAAGAGCTCGAGCGCCTTGGTGTAACCCC
CTCCGAGCTCATGCGGCAGGCTCTGCAATATGTCGCTGAGCGGGGCAAGCTACCCTTCAGGCCAGTCCTGATGACCGAGG
AAGATGAAGACCTCATTGCTACCGTACAAGAGCGGCTTGCTTCTGCGAAGCGCGTGAGGGTCCAGCTGGATGACCTATAG