Detailed information of TA system TA079212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 1315921..1316585
• Replicon: chromosome
• Accession: NZ_CP022560
• Organism: Pseudomonas putida strain B1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CHR26_RS06285
• Protein ID: WP_186506740.1
• Coordinates: 1316436..1316585 (-)
• Length: 50 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CHR26_RS06280
• Protein ID: WP_145913471.1
• Coordinates: 1315921..1316394 (-)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHR26_RS06265	1311776..1312654	+	879	WP_145913469.1	hypothetical protein	-
CHR26_RS06270	1312641..1313777	+	1137	WP_145913470.1	hypothetical protein	-
CHR26_RS06275	1314088..1315696	-	1609	Protein_1177	diguanylate cyclase	-
CHR26_RS06280	1315921..1316394	-	474	WP_145913471.1	transcriptional regulator	Antitoxin
CHR26_RS06285	1316436..1316585	-	150	WP_186506740.1	type II toxin-antitoxin system HigB family toxin	Toxin
CHR26_RS06290	1316736..1316942	+	207	WP_024717587.1	DUF3079 domain-containing protein	-
CHR26_RS06295	1317070..1319367	-	2298	WP_145913472.1	TonB-dependent siderophore receptor	-
CHR26_RS06300	1319508..1320230	+	723	WP_145913473.1	response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (11-43)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5867.87 Da
• Isoelectric Point: 10.5577

>T79212 WP_186506740.1 NZ_CP022560:c1316585-1316436 [Pseudomonas putida]
MLGRRDNTMRVKKNECRLIAWLNARTGTLYIKAILSHAEYDKWCRSDIR

Nucleotide

• Length: 150 bp

>T79212 NZ_CP022560:c1316585-1316436 [Pseudomonas putida]
ATGTTGGGGCGCCGCGACAACACAATGCGGGTCAAGAAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGG
CACTCTCTACATCAAGGCCATTTTGTCGCACGCCGAGTACGACAAATGGTGCCGAAGCGATATCAGGTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17231.64 Da
• Isoelectric Point: 4.4982

>AT79212 WP_145913471.1 NZ_CP022560:c1316394-1315921 [Pseudomonas putida]
MKRDKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELEARMALIDELYSHADTEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSETLNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT79212 NZ_CP022560:c1316394-1315921 [Pseudomonas putida]
ATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCCTGAACAC
TCAGATGGCAACCATGGCCCAGCTTCTGGCCGCCTGCCATGACCCCATCAAGGACGATGCCGAGCTTGAGGCACGCATGG
CATTGATCGACGAGCTCTACTCCCATGCGGACACTGAAGGCCACGCTGCGGCACGCTTTGCGGAGATGGTGGCGGACCGC
GTCTACGAGTATGAGAGTGAGTCTGTATTGGTGCCGTTCGCGAGCCAGGCCGAAGCGTTGGCCTTTCTGCTGTCGGACAG
GGGGGTGAAGCAGAAGGACCTGTCGGCGATAGCGACCCAGAGCGCGGTATCAGAGACTCTGAACAACAAGCGGAAGATGA
CGGTTGCCCAGATAAAGGGCTTTGCTGAGTTTTTCAGCGTACCGGTCGAGTTCTTCATGCATGGGGTGGTTTGA