Detailed information of TA system TA079210

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2453457..2454094
• Replicon: chromosome
• Accession: NZ_CP022556
• Organism: Bacillus velezensis strain NJAU-Z9

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: CF389_RS11965
• Protein ID: WP_003156187.1
• Coordinates: 2453457..2453807 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: CF389_RS11970
• Protein ID: WP_003156188.1
• Coordinates: 2453813..2454094 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF389_RS11925	2448497..2449099	-	603	WP_003156170.1	SpoIIE family protein phosphatase	-
CF389_RS11930	2449099..2449887	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
CF389_RS11935	2449853..2450335	-	483	WP_003156173.1	anti-sigma B factor RsbW	-
CF389_RS11940	2450332..2450661	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
CF389_RS11945	2450725..2451732	-	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
CF389_RS11950	2451744..2452145	-	402	WP_003156178.1	anti-sigma regulatory factor	-
CF389_RS11955	2452148..2452513	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
CF389_RS11960	2452518..2453339	-	822	WP_003156182.1	STAS domain-containing protein	-
CF389_RS11965	2453457..2453807	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CF389_RS11970	2453813..2454094	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
CF389_RS11975	2454214..2455383	-	1170	WP_003156189.1	alanine racemase	-
CF389_RS11980	2455500..2456507	-	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
CF389_RS11985	2456672..2457037	-	366	WP_003156192.1	holo-ACP synthase	-
CF389_RS11990	2457130..2457729	+	600	WP_003156193.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T79210 WP_003156187.1 NZ_CP022556:c2453807-2453457 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T79210 NZ_CP022556:c2453807-2453457 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT79210 WP_003156188.1 NZ_CP022556:c2454094-2453813 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT79210 NZ_CP022556:c2454094-2453813 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5