Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1701044..1701961 Replicon chromosome
Accession NZ_CP022556
Organism Bacillus velezensis strain NJAU-Z9

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A8LGT8
Locus tag CF389_RS07985 Protein ID WP_003154806.1
Coordinates 1701044..1701790 (+) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag CF389_RS07990 Protein ID WP_003154807.1
Coordinates 1701791..1701961 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CF389_RS07965 1696436..1697386 - 951 WP_003154799.1 ring-cleaving dioxygenase -
CF389_RS07970 1697709..1699025 + 1317 WP_003154801.1 amino acid permease -
CF389_RS07975 1699311..1699928 + 618 WP_003154804.1 DUF47 domain-containing protein -
CF389_RS07980 1699941..1700939 + 999 WP_003154805.1 inorganic phosphate transporter -
CF389_RS07985 1701044..1701790 + 747 WP_003154806.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
CF389_RS07990 1701791..1701961 + 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
CF389_RS07995 1702219..1703097 - 879 WP_003154811.1 N-acetylmuramoyl-L-alanine amidase -
CF389_RS08000 1703111..1703374 - 264 WP_003154813.1 phage holin -
CF389_RS08005 1703388..1703651 - 264 WP_003154815.1 hemolysin XhlA family protein -
CF389_RS08010 1703703..1704464 - 762 WP_003154817.1 hypothetical protein -
CF389_RS08015 1704521..1704718 - 198 WP_003154819.1 XkdX family protein -
CF389_RS08020 1704724..1705095 - 372 WP_003154820.1 hypothetical protein -
CF389_RS08025 1705108..1706730 - 1623 WP_003154821.1 pyocin knob domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1674871..1749015 74144


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29034.50 Da        Isoelectric Point: 4.6947

>T79209 WP_003154806.1 NZ_CP022556:1701044-1701790 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T79209 NZ_CP022556:1701044-1701790 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
AGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTTCAGGACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT79209 WP_003154807.1 NZ_CP022556:1701791-1701961 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT79209 NZ_CP022556:1701791-1701961 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGATAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A8LGT8

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References