Detailed information of TA system TA079201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 13234..13877
• Replicon: plasmid pMS7884B
• Accession: NZ_CP022534
• Organism: Enterobacter hormaechei strain MS7884A

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: MS7884_RS25510
• Protein ID: WP_016236302.1
• Coordinates: 13461..13877 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: MS7884_RS25505
• Protein ID: WP_001261282.1
• Coordinates: 13234..13464 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS7884_RS25470	9333..9959	+	627	WP_008502208.1	ParA family plasmid-partitioning AAA ATPase	-
MS7884_RS25475	9956..10261	+	306	WP_071530977.1	hypothetical protein	-
MS7884_RS25480	10342..11118	-	777	WP_033488148.1	site-specific integrase	-
MS7884_RS25485	11118..11936	-	819	WP_033488147.1	hypothetical protein	-
MS7884_RS25490	11985..12314	-	330	WP_033488146.1	hypothetical protein	-
MS7884_RS25495	12360..12662	-	303	WP_033488145.1	hypothetical protein	-
MS7884_RS25500	12817..13277	-	461	Protein_17	hypothetical protein	-
MS7884_RS25505	13234..13464	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MS7884_RS25510	13461..13877	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
MS7884_RS25515	13951..14187	+	237	WP_001348075.1	AAA family ATPase	-
MS7884_RS25520	14234..14938	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
MS7884_RS25525	14992..16089	+	1098	Protein_22	IS66 family transposase	-
MS7884_RS25530	16340..16903	-	564	WP_001097412.1	chlorite dismutase family protein	-
MS7884_RS25535	16927..17301	-	375	WP_001348195.1	cupin domain-containing protein	-
MS7884_RS25540	17366..17929	-	564	WP_001515734.1	transposase	-
MS7884_RS25550	18172..18507	+	336	WP_000046891.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..126208	126208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T79201 WP_016236302.1 NZ_CP022534:13461-13877 [Enterobacter hormaechei]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T79201 NZ_CP022534:13461-13877 [Enterobacter hormaechei]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT79201 WP_001261282.1 NZ_CP022534:13234-13464 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT79201 NZ_CP022534:13234-13464 [Enterobacter hormaechei]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3