Detailed information of TA system TA079181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1549614..1550251
• Replicon: chromosome
• Accession: NZ_CP022531
• Organism: Bacillus velezensis strain TB1501

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: CG798_RS08055
• Protein ID: WP_003156187.1
• Coordinates: 1549901..1550251 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: CG798_RS08050
• Protein ID: WP_003156188.1
• Coordinates: 1549614..1549895 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CG798_RS08030	1545979..1546578	-	600	WP_015239195.1	rhomboid family intramembrane serine protease	-
CG798_RS08035	1546671..1547036	+	366	WP_094031521.1	holo-ACP synthase	-
CG798_RS08040	1547201..1548208	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
CG798_RS08045	1548325..1549494	+	1170	WP_025284272.1	alanine racemase	-
CG798_RS08050	1549614..1549895	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
CG798_RS08055	1549901..1550251	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CG798_RS08060	1550369..1551190	+	822	WP_003156182.1	STAS domain-containing protein	-
CG798_RS08065	1551195..1551560	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
CG798_RS08070	1551563..1551964	+	402	WP_003156178.1	anti-sigma regulatory factor	-
CG798_RS08075	1551976..1552983	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
CG798_RS08080	1553047..1553376	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
CG798_RS08085	1553373..1553855	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
CG798_RS08090	1553821..1554609	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
CG798_RS08095	1554609..1555211	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T79181 WP_003156187.1 NZ_CP022531:1549901-1550251 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T79181 NZ_CP022531:1549901-1550251 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT79181 WP_003156188.1 NZ_CP022531:1549614-1549895 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT79181 NZ_CP022531:1549614-1549895 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5