Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 3559422..3560030 Replicon chromosome
Accession NZ_CP022525
Organism Pseudomonas aeruginosa strain Ocean-1175

Toxin (Protein)


Gene name PfiT Uniprot ID A0A509JBR4
Locus tag CGU46_RS16955 Protein ID WP_031638390.1
Coordinates 3559683..3560030 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag CGU46_RS16950 Protein ID WP_003114155.1
Coordinates 3559422..3559673 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CGU46_RS16930 3555060..3555410 + 351 WP_003133726.1 DUF2523 domain-containing protein -
CGU46_RS16935 3555412..3556674 + 1263 WP_033953311.1 hypothetical protein -
CGU46_RS16940 3556933..3558225 + 1293 WP_003123045.1 hypothetical protein -
CGU46_RS16945 3558225..3559208 + 984 WP_034014422.1 tyrosine-type recombinase/integrase -
CGU46_RS16950 3559422..3559673 + 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CGU46_RS16955 3559683..3560030 + 348 WP_031638390.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CGU46_RS16960 3560339..3561247 - 909 WP_003141085.1 LysR family transcriptional regulator -
CGU46_RS16965 3561480..3562631 - 1152 WP_012761372.1 trypsin-like peptidase domain-containing protein -
CGU46_RS16970 3562656..3563621 - 966 WP_003141083.1 M48 family metalloprotease -
CGU46_RS16975 3563665..3564096 - 432 WP_019485901.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3545311..3572461 27150


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13018.79 Da        Isoelectric Point: 4.4212

>T79168 WP_031638390.1 NZ_CP022525:3559683-3560030 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLVPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T79168 NZ_CP022525:3559683-3560030 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGTTCCATTCCA
AGGTGAACAGGCTGCATTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT79168 WP_003114155.1 NZ_CP022525:3559422-3559673 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT79168 NZ_CP022525:3559422-3559673 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGATCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A509JBR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References