Detailed information of TA system TA079151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafQ-relB/YafQ-RelB
• Location: 20640..21172
• Replicon: plasmid pPant2
• Accession: NZ_CP022518
• Organism: Pantoea vagans strain FBS135

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: B9D02_RS21750
• Protein ID: WP_197711780.1
• Coordinates: 20894..21172 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: B9D02_RS21745
• Protein ID: WP_110412005.1
• Coordinates: 20640..20897 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9D02_RS21720	15856..16329	-	474	WP_110412001.1	hypothetical protein	-
B9D02_RS21725	16442..17131	-	690	WP_110412002.1	NYN domain-containing protein	-
B9D02_RS21730	17741..18637	-	897	WP_110412003.1	DMT family transporter	-
B9D02_RS21735	18691..19290	+	600	WP_110412004.1	TetR/AcrR family transcriptional regulator	-
B9D02_RS21740	19392..20424	+	1033	Protein_18	translesion error-prone DNA polymerase V subunit UmuC	-
B9D02_RS21745	20640..20897	+	258	WP_110412005.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
B9D02_RS21750	20894..21172	+	279	WP_197711780.1	type II toxin-antitoxin system YafQ family toxin	Toxin
B9D02_RS21755	21362..22101	-	740	Protein_21	HNH endonuclease	-
B9D02_RS23690	22098..22454	-	357	WP_128755149.1	hypothetical protein	-
B9D02_RS21765	22408..22944	-	537	WP_110412008.1	ATP-binding protein	-
B9D02_RS21770	23015..23311	-	297	WP_164913102.1	AAA family ATPase	-
B9D02_RS23695	23520..23723	-	204	WP_128755150.1	hypothetical protein	-
B9D02_RS21775	23808..24599	+	792	WP_110412010.1	tyrosine-type recombinase/integrase	-
B9D02_RS21780	24932..25588	+	657	WP_110412011.1	AAA family ATPase	-
B9D02_RS21785	25633..25902	+	270	WP_110412012.1	ribbon-helix-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..196103	196103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (7-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10825.50 Da
• Isoelectric Point: 9.3828

>T79151 WP_197711780.1 NZ_CP022518:20894-21172 [Pantoea vagans]
MMLTPVQASAFKRDTKRQKKRGKDMTKLKTLITLLVKEMEIPPEYEDHPLQGDWRGYRDAHMEGDWILIYKVEGTDLKLA
RTGTHQDIFSNY

Nucleotide

• Length: 279 bp

>T79151 NZ_CP022518:20894-21172 [Pantoea vagans]
TTGATGCTAACACCGGTACAAGCGAGTGCTTTTAAAAGAGATACAAAACGACAGAAAAAACGCGGCAAGGATATGACCAA
GCTGAAGACGTTGATAACGCTGCTGGTCAAAGAAATGGAAATACCGCCAGAGTATGAAGATCACCCCTTACAGGGAGACT
GGCGAGGCTACCGCGATGCGCATATGGAAGGTGACTGGATACTGATTTATAAAGTTGAAGGTACAGATCTCAAACTCGCA
AGAACCGGAACGCATCAGGACATTTTTAGCAACTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9434.81 Da
• Isoelectric Point: 5.1333

>AT79151 WP_110412005.1 NZ_CP022518:20640-20897 [Pantoea vagans]
MIADALVRARIPRDTKDRAVAALDKMGLSTSDVIRLVMMRVAEEGRLPFEVNTPNSATRRAIDELENGRGKRFDTADALF
DDMGI

Nucleotide

• Length: 258 bp

>AT79151 NZ_CP022518:20640-20897 [Pantoea vagans]
ATGATCGCAGATGCACTGGTAAGAGCGCGTATTCCGCGGGATACGAAAGATCGAGCTGTCGCTGCACTGGACAAGATGGG
CCTCAGCACTTCGGATGTTATCCGCCTGGTAATGATGCGCGTAGCGGAAGAAGGCCGTCTGCCCTTTGAGGTTAATACCC
CTAATTCTGCTACTCGCCGTGCTATAGACGAACTGGAAAATGGTCGCGGTAAACGTTTCGATACTGCTGATGCCTTGTTC
GATGATATGGGGATTTGA