Detailed information of TA system TA079131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 2275647..2276342
• Replicon: chromosome
• Accession: NZ_CP022488
• Organism: Enterococcus faecalis ARO1/DG

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: CG806_RS12510
• Protein ID: WP_002367289.1
• Coordinates: 2275998..2276342 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: CG806_RS12505
• Protein ID: WP_002367288.1
• Coordinates: 2275647..2275979 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CG806_RS12450	2270967..2271746	-	780	WP_002367278.1	DnaD domain protein	-
CG806_RS12455	2271761..2272576	-	816	WP_002367279.1	PD-(D/E)XK nuclease-like domain-containing protein	-
CG806_RS12460	2272539..2273504	-	966	WP_010717378.1	recombinase RecT	-
CG806_RS12470	2273605..2273829	-	225	WP_002367281.1	hypothetical protein	-
CG806_RS12475	2273826..2274125	-	300	WP_010717380.1	hypothetical protein	-
CG806_RS12480	2274192..2274371	-	180	WP_002367283.1	hypothetical protein	-
CG806_RS12485	2274406..2274675	-	270	WP_002367284.1	hypothetical protein	-
CG806_RS12490	2274691..2274867	-	177	WP_002367285.1	helix-turn-helix domain-containing protein	-
CG806_RS12495	2274939..2275160	+	222	WP_002367286.1	hypothetical protein	-
CG806_RS12500	2275157..2275348	-	192	WP_002367287.1	hypothetical protein	-
CG806_RS12505	2275647..2275979	+	333	WP_002367288.1	helix-turn-helix domain-containing protein	Antitoxin
CG806_RS12510	2275998..2276342	+	345	WP_002367289.1	ImmA/IrrE family metallo-endopeptidase	Toxin
CG806_RS12515	2276368..2276601	-	234	WP_002367290.1	hypothetical protein	-
CG806_RS12520	2276691..2277374	+	684	WP_129194668.1	hypothetical protein	-
CG806_RS12525	2277551..2278732	+	1182	WP_002367292.1	site-specific integrase	-
CG806_RS12530	2278835..2278984	-	150	WP_002356321.1	50S ribosomal protein L33	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2243373..2278732	35359

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-59)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13668.65 Da
• Isoelectric Point: 5.2241

>T79131 WP_002367289.1 NZ_CP022488:2275998-2276342 [Enterococcus faecalis ARO1/DG]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPSEQEEAIYHELKHVKDHVDIMELYKIPVFRSKMESEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEIYLK

Nucleotide

• Length: 345 bp

>T79131 NZ_CP022488:2275998-2276342 [Enterococcus faecalis ARO1/DG]
ATGAAAAGTATCAAAGAGTTGGTAGAAGAATATAATGTGGAGTTAGTTTTTACTACTTTGAACAAACGCGCATGTTTCGA
CCCTACCTACGGTATCATATTTGTAAATCAAAATTTAACACCATCAGAACAAGAAGAAGCAATATATCACGAGTTAAAGC
ACGTAAAAGACCATGTGGATATAATGGAATTGTATAAAATTCCTGTTTTTCGTTCTAAGATGGAATCCGAAGCAGAACAA
TATATGTTTAGAAGCTTAATCGAAAAATATGAAGGACAATACAATTACTCAAATGTTATAGCTCATTACAACTTAAAAAT
GGGACAAGAAATTTATTTGAAATAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12676.15 Da
• Isoelectric Point: 4.7492

>AT79131 WP_002367288.1 NZ_CP022488:2275647-2275979 [Enterococcus faecalis ARO1/DG]
MNLLERIKKLAKDRDVSIYQLEEEINIGRNTIYQWNKRTPSSDKLEAVANYFNVSVDYLLGRTENPNQAGAKPSDDLNDV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT79131 NZ_CP022488:2275647-2275979 [Enterococcus faecalis ARO1/DG]
ATGAACCTTTTGGAACGTATAAAAAAACTAGCAAAAGATAGAGATGTGTCTATTTATCAGCTTGAAGAAGAAATAAATAT
TGGACGAAATACTATTTATCAATGGAATAAGCGAACACCTTCTTCGGATAAACTTGAAGCTGTAGCCAATTATTTTAATG
TTTCTGTTGATTATTTATTAGGTCGTACTGAAAATCCAAACCAAGCTGGTGCTAAACCATCTGACGATTTAAATGATGTA
CTGGATAACGTCATGAGTTTTGACGGTGAACCACTTGATGATCATGACAGAGAAGTTATTCGTGCATATTTAAAGGGTAG
ATTCGGGAAATAA