Detailed information of TA system TA079128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 73297..73950
• Replicon: chromosome
• Accession: NZ_CP022488
• Organism: Enterococcus faecalis ARO1/DG

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: R3IHS7
• Locus tag: CG806_RS01290
• Protein ID: WP_002367585.1
• Coordinates: 73768..73950 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: R3IHD2
• Locus tag: CG806_RS01285
• Protein ID: WP_010707936.1
• Coordinates: 73297..73734 (-)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CG806_RS01270	68570..69268	+	699	WP_002367581.1	N-acetylmannosamine-6-phosphate 2-epimerase	-
CG806_RS01275	69447..71786	+	2340	WP_025186725.1	alpha-glucosidase	-
CG806_RS01280	72333..73250	+	918	WP_002367583.1	helix-turn-helix domain-containing protein	-
CG806_RS01285	73297..73734	-	438	WP_010707936.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CG806_RS01290	73768..73950	-	183	WP_002367585.1	type II toxin-antitoxin system HicA family toxin	Toxin
CG806_RS01295	74056..74709	-	654	WP_002356081.1	Crp/Fnr family transcriptional regulator	-
CG806_RS01300	74983..75876	+	894	WP_002367586.1	SDR family oxidoreductase	-
CG806_RS01305	75889..76461	+	573	WP_025186726.1	alkaline shock response membrane anchor protein AmaP	-
CG806_RS01310	76474..76665	+	192	WP_002356087.1	DUF2273 domain-containing protein	-
CG806_RS01315	76678..77190	+	513	WP_002383483.1	Asp23/Gls24 family envelope stress response protein	-
CG806_RS01320	77249..77809	+	561	WP_002356090.1	Asp23/Gls24 family envelope stress response protein	-
CG806_RS01325	77833..78075	+	243	WP_002356092.1	GlsB/YeaQ/YmgE family stress response membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (11-71)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6633.06 Da
• Isoelectric Point: 10.8756

>T79128 WP_002367585.1 NZ_CP022488:c73950-73768 [Enterococcus faecalis ARO1/DG]
MPMTQKQMVKLLKKHGWLPTDGGKGSHIKMEMSGKRPIIIPHGELKKGTEIGILKEAGLK

Nucleotide

• Length: 183 bp

>T79128 NZ_CP022488:c73950-73768 [Enterococcus faecalis ARO1/DG]
ATGCCAATGACACAAAAACAAATGGTCAAATTGCTAAAAAAGCACGGTTGGTTACCAACCGATGGTGGAAAAGGCTCCCA
TATAAAAATGGAGATGTCTGGCAAAAGACCCATCATTATCCCACACGGAGAATTGAAAAAAGGAACCGAAATAGGGATTT
TAAAAGAGGCAGGGCTGAAATAG

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16267.27 Da
• Isoelectric Point: 4.2758

>AT79128 WP_010707936.1 NZ_CP022488:c73734-73297 [Enterococcus faecalis ARO1/DG]
MLISYPACFYSVENGYYVYFPDIGGSGTQGDTIPDAIAMASDYLGMMLSDDIEHQRTIHQPSPINTLSLEDNNPFRDDDD
FNFNLADSFVSMVNVELNDYLGTNTLVKKTVTIPKWSDELGKKNKLNFSKTLTDAIVEKSLHVLK

Nucleotide

• Length: 438 bp

>AT79128 NZ_CP022488:c73734-73297 [Enterococcus faecalis ARO1/DG]
ATGCTAATTAGTTATCCTGCATGTTTCTACAGTGTCGAGAATGGTTACTATGTTTATTTCCCAGATATAGGTGGTTCTGG
TACGCAAGGAGATACAATTCCTGATGCAATTGCGATGGCTTCCGATTATTTAGGTATGATGTTATCTGATGATATTGAAC
ATCAAAGAACAATCCACCAGCCATCTCCTATCAATACGTTGTCATTAGAAGATAATAATCCCTTTAGAGATGACGATGAC
TTCAATTTTAACCTTGCAGACTCGTTTGTTTCTATGGTAAATGTAGAACTAAATGATTATTTAGGAACAAATACGTTAGT
CAAAAAAACCGTCACCATTCCCAAGTGGTCTGATGAACTCGGGAAAAAAAATAAATTGAATTTTTCTAAAACCTTGACCG
ATGCCATCGTTGAAAAGTCGCTACATGTTTTAAAGTAG

Structures

Toxin

• AlphaFold DB: R3IHS7

Antitoxin

• AlphaFold DB: R3IHD2