Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 50862..51433 Replicon plasmid pARO1.1
Accession NZ_CP022484
Organism Enterococcus faecalis ARO1/DG

Toxin (Protein)


Gene name mazF Uniprot ID R3HR30
Locus tag CG806_RS00305 Protein ID WP_001278770.1
Coordinates 50862..51203 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6B1XRC3
Locus tag CG806_RS00310 Protein ID WP_000958277.1
Coordinates 51203..51433 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CG806_RS00285 47136..47549 - 414 WP_002367777.1 hypothetical protein -
CG806_RS00290 48028..48708 + 681 WP_002367779.1 IS6 family transposase -
CG806_RS00295 48942..49544 - 603 WP_002367780.1 Fic family protein -
CG806_RS00300 49812..50750 - 939 WP_002367782.1 hypothetical protein -
CG806_RS00305 50862..51203 - 342 WP_001278770.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CG806_RS00310 51203..51433 - 231 WP_000958277.1 hypothetical protein Antitoxin
CG806_RS00315 51637..52257 + 621 WP_002367784.1 recombinase family protein -
CG806_RS00320 52247..52561 + 315 WP_002367785.1 hypothetical protein -
CG806_RS00330 52921..53115 + 195 WP_002367787.1 hypothetical protein -
CG806_RS00335 53127..53318 + 192 WP_002367788.1 hypothetical protein -
CG806_RS00340 53488..53703 + 216 WP_002367791.1 leucocin A/sakacin P family class II bacteriocin -
CG806_RS00345 53704..54045 + 342 WP_002367792.1 bacteriocin immunity protein -
CG806_RS00350 54461..54979 + 519 WP_002367793.1 hypothetical protein -
CG806_RS00360 55234..55320 + 87 WP_012881081.1 type I toxin-antitoxin system Fst family toxin -
CG806_RS00365 55577..55873 + 297 WP_002367795.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(L) / tet(M) - 1..65180 65180
- flank IS/Tn - - 48028..48708 680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13220.44 Da        Isoelectric Point: 8.8595

>T79123 WP_001278770.1 NZ_CP022484:c51203-50862 [Enterococcus faecalis ARO1/DG]
MSDEKKYIPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T79123 NZ_CP022484:c51203-50862 [Enterococcus faecalis ARO1/DG]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGGAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACAATGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCCACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGATTTTACAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8536.45 Da        Isoelectric Point: 4.2412

>AT79123 WP_000958277.1 NZ_CP022484:c51433-51203 [Enterococcus faecalis ARO1/DG]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWYDIEPGGRELF

Download         Length: 231 bp

>AT79123 NZ_CP022484:c51433-51203 [Enterococcus faecalis ARO1/DG]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAACAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGATCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGTATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3HR30

Antitoxin

Source ID Structure
AlphaFold DB A0A6B1XRC3

References