Detailed information of TA system TA079122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 38186..38805
• Replicon: plasmid pARO1.1
• Accession: NZ_CP022484
• Organism: Enterococcus faecalis ARO1/DG

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: R3H4V2
• Locus tag: CG806_RS00250
• Protein ID: WP_000241511.1
• Coordinates: 38443..38805 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3H5D1
• Locus tag: CG806_RS00245
• Protein ID: WP_000245205.1
• Coordinates: 38186..38449 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CG806_RS00225	33445..34758	+	1314	WP_002383787.1	hypothetical protein	-
CG806_RS00230	35140..35781	+	642	WP_000406546.1	hypothetical protein	-
CG806_RS00240	37032..37688	+	657	WP_000411487.1	hypothetical protein	-
CG806_RS00245	38186..38449	+	264	WP_000245205.1	PbsX family transcriptional regulator	Antitoxin
CG806_RS00250	38443..38805	+	363	WP_000241511.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CG806_RS00255	38870..39832	-	963	WP_002367770.1	LacI family DNA-binding transcriptional regulator	-
CG806_RS00260	39834..41273	-	1440	WP_002367771.1	sucrose-6-phosphate hydrolase	-
CG806_RS00265	41464..43395	+	1932	WP_002367772.1	sucrose-specific PTS transporter subunit IIBC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(L) / tet(M)	-	1..65180	65180

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13585.78 Da
• Isoelectric Point: 9.6107

>T79122 WP_000241511.1 NZ_CP022484:38443-38805 [Enterococcus faecalis ARO1/DG]
MVKVPHQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVIVAPISSTKRNYPLYVSINPSYGMKTSGKVLLDQLT
TIDYEARQCVFLETAHEKLIDELLLKVRTVFQKVNKTNKF

Nucleotide

• Length: 363 bp

>T79122 NZ_CP022484:38443-38805 [Enterococcus faecalis ARO1/DG]
ATGGTAAAAGTTCCTCATCAAGGTGATATTTTATTGTTGAATACGGCTCCTAGATCAGGACATGAACAAACAGGTAAGAG
ACCGTATATTGTTTTAAGTCATGATATTATTGCAGATTACAGCAATGTTGTTATTGTAGCTCCTATATCAAGTACAAAAA
GAAACTATCCTTTATATGTTTCTATTAATCCTTCTTACGGAATGAAAACATCAGGTAAAGTATTATTAGATCAATTAACC
ACCATCGATTATGAGGCTAGACAATGCGTGTTTTTAGAAACAGCACACGAAAAATTAATCGATGAATTATTGCTAAAAGT
TAGAACCGTATTTCAAAAAGTAAATAAAACAAATAAATTTTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10059.46 Da
• Isoelectric Point: 5.1585

>AT79122 WP_000245205.1 NZ_CP022484:38186-38449 [Enterococcus faecalis ARO1/DG]
MVMLSIKKWGNSNGLRLPKSVMEYLQIHTEDKVKITQEESNGKKRLIIEAVDSDNDLTIEQLFENYKEEKVHVTIQDLGN
AVGNEKW

Nucleotide

• Length: 264 bp

>AT79122 NZ_CP022484:38186-38449 [Enterococcus faecalis ARO1/DG]
ATGGTAATGTTATCAATAAAAAAATGGGGCAATAGTAACGGTCTTAGACTTCCTAAATCTGTGATGGAATATCTCCAAAT
TCATACAGAAGATAAAGTGAAAATAACACAGGAGGAATCGAATGGTAAAAAACGATTAATTATTGAAGCTGTGGATTCTG
ATAATGATTTAACAATTGAACAATTGTTTGAAAATTATAAAGAAGAGAAGGTGCATGTAACTATTCAAGATTTAGGAAAT
GCGGTAGGAAATGAAAAATGGTAA

Structures

Toxin

• AlphaFold DB: R3H4V2

Antitoxin

• AlphaFold DB: R3H5D1