Detailed information of TA system TA079112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4280308..4280894
• Replicon: chromosome
• Accession: NZ_CP022478
• Organism: Pseudomonas aeruginosa strain LW

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: CEK59_RS20330
• Protein ID: WP_003120987.1
• Coordinates: 4280595..4280894 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CEK59_RS20325
• Protein ID: WP_003448662.1
• Coordinates: 4280308..4280598 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEK59_RS20310	4275892..4277781	+	1890	WP_023101908.1	hypothetical protein	-
CEK59_RS20315	4277778..4279754	+	1977	WP_031691363.1	DEAD/DEAH box helicase	-
CEK59_RS20320	4279893..4280237	+	345	WP_023082045.1	hypothetical protein	-
CEK59_RS20325	4280308..4280598	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
CEK59_RS20330	4280595..4280894	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CEK59_RS20335	4281096..4282220	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
CEK59_RS20340	4282220..4283929	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
CEK59_RS20345	4283933..4285258	+	1326	WP_126160157.1	type 4b pilus protein PilO2	-
CEK59_RS20350	4285248..4285781	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4249539..4333185	83646

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T79112 WP_003120987.1 NZ_CP022478:c4280894-4280595 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T79112 NZ_CP022478:c4280894-4280595 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT79112 WP_003448662.1 NZ_CP022478:c4280598-4280308 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT79112 NZ_CP022478:c4280598-4280308 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73