Detailed information of TA system TA079096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 6480699..6481264
• Replicon: chromosome
• Accession: NZ_CP022464
• Organism: Enterocloster bolteae strain ATCC BAA-613

Toxin (Protein)

• Gene name: relE
• Uniprot ID: C7H6P5
• Locus tag: CGC65_RS30210
• Protein ID: WP_002596328.1
• Coordinates: 6480974..6481264 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C7H6P4
• Locus tag: CGC65_RS30205
• Protein ID: WP_005924829.1
• Coordinates: 6480699..6480977 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CGC65_RS30165	6476033..6476338	-	306	WP_002569240.1	hypothetical protein	-
CGC65_RS30170	6476502..6476642	-	141	WP_007037419.1	membrane protein	-
CGC65_RS30175	6476861..6477676	-	816	Protein_5862	type IV secretory system conjugative DNA transfer family protein	-
CGC65_RS30180	6477770..6478135	-	366	WP_014080225.1	hypothetical protein	-
CGC65_RS30185	6478213..6478407	-	195	WP_005933543.1	transposon-encoded TnpW family protein	-
CGC65_RS30190	6478451..6479314	-	864	WP_005933545.1	ATP-binding protein	-
CGC65_RS30195	6479311..6480060	-	750	WP_005933547.1	phage replisome organizer N-terminal domain-containing protein	-
CGC65_RS30200	6480174..6480572	-	399	WP_005933550.1	cysteine-rich VLP domain-containing protein	-
CGC65_RS30205	6480699..6480977	+	279	WP_005924829.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CGC65_RS30210	6480974..6481264	+	291	WP_002596328.1	type II toxin-antitoxin system YafQ family toxin	Toxin
CGC65_RS30215	6481372..6482817	-	1446	WP_007037415.1	MobA/MobL family protein	-
CGC65_RS30220	6483004..6483294	-	291	WP_005933556.1	DUF3847 domain-containing protein	-
CGC65_RS30225	6483323..6483784	-	462	WP_035394327.1	sigma-70 family RNA polymerase sigma factor	-
CGC65_RS30230	6483795..6484247	-	453	WP_005933558.1	hypothetical protein	-
CGC65_RS30235	6484351..6484764	-	414	WP_035394329.1	hypothetical protein	-
CGC65_RS30240	6485218..6485679	-	462	WP_038259777.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-94)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-90)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11112.87 Da
• Isoelectric Point: 8.0630

>T79096 WP_002596328.1 NZ_CP022464:6480974-6481264 [Enterocloster bolteae]
MKETKYTVKYTTSFKKDYKRAIKRGLKIELLEQVVALLAMGEPLPDKNRDHDLSGDWAGHRECHILPDWLLVYRIEDDVL
VLTLARTGTHSDLFGK

Nucleotide

• Length: 291 bp

>T79096 NZ_CP022464:6480974-6481264 [Enterocloster bolteae]
ATGAAAGAAACCAAATATACCGTCAAGTACACGACCAGTTTCAAAAAGGACTACAAACGAGCCATCAAGCGTGGCTTGAA
GATTGAGCTGCTGGAGCAGGTCGTAGCATTGCTGGCAATGGGCGAACCGCTGCCGGATAAGAACCGTGACCATGACCTGT
CCGGCGATTGGGCAGGTCATCGGGAATGTCATATTCTCCCGGACTGGCTGCTTGTCTATCGCATAGAGGATGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10247.66 Da
• Isoelectric Point: 4.4692

>AT79096 WP_005924829.1 NZ_CP022464:6480699-6480977 [Enterocloster bolteae]
MAGNTTNISIRMDADLKAQADALFTELGMNLTTAFNIFVRQSLREGGIPFEVRLEQPNKETVAAMLEAERIAKDPSVKGY
NDLDELFADLKR

Nucleotide

• Length: 279 bp

>AT79096 NZ_CP022464:6480699-6480977 [Enterocloster bolteae]
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCGCAGGCGGACGCACTGTTTACGGAACT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAAGAAACGGTTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGCTAC
AATGACCTTGACGAGTTATTTGCTGACCTGAAAAGATGA

Structures

Toxin

• AlphaFold DB: A0A1M4XBM1

Antitoxin

• AlphaFold DB: A0A1M4XB19