Detailed information of TA system TA079085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2464084..2464921
• Replicon: chromosome
• Accession: NZ_CP022457
• Organism: Shigella sonnei strain 2015C-3566

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: B9128_RS13340
• Protein ID: WP_000227784.1
• Coordinates: 2464084..2464626 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: B9128_RS13345
• Protein ID: WP_001297137.1
• Coordinates: 2464610..2464921 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9128_RS13300 (2459148)	2459148..2459639	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
B9128_RS13305 (2459767)	2459767..2461131	-	1365	WP_001000971.1	MFS transporter	-
B9128_RS13315 (2461250)	2461250..2461947	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
B9128_RS13320 (2462316)	2462316..2462714	+	399	Protein_2378	SEL1-like repeat protein	-
B9128_RS13335 (2463462)	2463462..2464028	+	567	Protein_2380	SEL1-like repeat protein	-
B9128_RS13340 (2464084)	2464084..2464626	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
B9128_RS13345 (2464610)	2464610..2464921	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
B9128_RS13350 (2465106)	2465106..2465996	-	891	WP_000971327.1	heme o synthase	-
B9128_RS13355 (2466008)	2466008..2466337	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
B9128_RS13360 (2466337)	2466337..2466951	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
B9128_RS13365 (2466941)	2466941..2468932	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
B9128_RS13370 (2468954)	2468954..2469901	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T79085 WP_000227784.1 NZ_CP022457:c2464626-2464084 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T79085 NZ_CP022457:c2464626-2464084 [Shigella sonnei]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT79085 WP_001297137.1 NZ_CP022457:c2464921-2464610 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT79085 NZ_CP022457:c2464921-2464610 [Shigella sonnei]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3