Detailed information of TA system TA079073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4261242..4262079
• Replicon: chromosome
• Accession: NZ_CP022455
• Organism: Shigella sonnei strain 2015C-3794

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: B9129_RS23465
• Protein ID: WP_000227784.1
• Coordinates: 4261242..4261784 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: B9129_RS23470
• Protein ID: WP_001297137.1
• Coordinates: 4261768..4262079 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9129_RS23425 (4256306)	4256306..4256797	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
B9129_RS23430 (4256925)	4256925..4258289	-	1365	WP_001000971.1	MFS transporter	-
B9129_RS23440 (4258408)	4258408..4259105	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
B9129_RS23445 (4259474)	4259474..4259872	+	399	Protein_4207	SEL1-like repeat protein	-
B9129_RS23460 (4260620)	4260620..4261186	+	567	Protein_4209	SEL1-like repeat protein	-
B9129_RS23465 (4261242)	4261242..4261784	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
B9129_RS23470 (4261768)	4261768..4262079	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
B9129_RS23475 (4262264)	4262264..4263154	-	891	WP_000971327.1	heme o synthase	-
B9129_RS23480 (4263166)	4263166..4263495	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
B9129_RS23485 (4263495)	4263495..4264109	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
B9129_RS23490 (4264099)	4264099..4266090	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
B9129_RS23495 (4266112)	4266112..4267059	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T79073 WP_000227784.1 NZ_CP022455:c4261784-4261242 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T79073 NZ_CP022455:c4261784-4261242 [Shigella sonnei]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT79073 WP_001297137.1 NZ_CP022455:c4262079-4261768 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT79073 NZ_CP022455:c4262079-4261768 [Shigella sonnei]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3