Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3575547..3576044 Replicon chromosome
Accession NZ_CP022455
Organism Shigella sonnei strain 2015C-3794

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag B9129_RS19880 Protein ID WP_001173343.1
Coordinates 3575871..3576044 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag B9129_RS19875 Protein ID WP_000398619.1
Coordinates 3575547..3575843 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9129_RS19850 (3572278) 3572278..3572997 - 720 WP_000611285.1 two-component system response regulator DcuR -
B9129_RS19855 (3572994) 3572994..3574625 - 1632 WP_001216495.1 sensor histidine kinase -
B9129_RS19860 (3574806) 3574806..3575036 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
B9129_RS19865 (3575048) 3575048..3575320 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
B9129_RS19875 (3575547) 3575547..3575843 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
B9129_RS19880 (3575871) 3575871..3576044 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
B9129_RS19885 (3576163) 3576163..3577680 - 1518 WP_001295086.1 lysine--tRNA ligase -
B9129_RS19890 (3577917) 3577917..3579374 - 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
B9129_RS19895 (3579433) 3579433..3579690 - 258 Protein_3554 lysine decarboxylase LdcC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T79071 WP_001173343.1 NZ_CP022455:3575871-3576044 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T79071 NZ_CP022455:3575871-3576044 [Shigella sonnei]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT79071 WP_000398619.1 NZ_CP022455:3575547-3575843 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT79071 NZ_CP022455:3575547-3575843 [Shigella sonnei]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References