Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 4568211..4568842 Replicon chromosome
Accession NZ_CP022444
Organism Klebsiella sp. LY

Toxin (Protein)


Gene name hicA Uniprot ID A0A1Q8YTV3
Locus tag CE636_RS25340 Protein ID WP_012542177.1
Coordinates 4568211..4568387 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A3Q9U6R4
Locus tag CE636_RS25345 Protein ID WP_017898984.1
Coordinates 4568435..4568842 (+) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CE636_RS25320 4563598..4564629 + 1032 WP_017898981.1 DUF968 domain-containing protein -
CE636_RS25325 4564642..4564983 + 342 WP_017898982.1 antitermination protein Q -
CE636_RS25330 4565008..4566042 - 1035 WP_071600377.1 DNA cytosine methyltransferase -
CE636_RS25335 4566062..4567882 - 1821 WP_017898983.1 ATP-binding protein -
CE636_RS25340 4568211..4568387 + 177 WP_012542177.1 type II toxin-antitoxin system HicA family toxin Toxin
CE636_RS25345 4568435..4568842 + 408 WP_017898984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CE636_RS25350 4569554..4569769 + 216 WP_017880269.1 class II holin family protein -
CE636_RS25355 4569769..4570266 + 498 WP_017898985.1 lysozyme -
CE636_RS25360 4570263..4570613 + 351 WP_017898986.1 hypothetical protein -
CE636_RS25380 4572363..4572725 + 363 WP_017898990.1 HNH endonuclease -
CE636_RS25385 4572677..4572994 + 318 WP_014228902.1 hypothetical protein -
CE636_RS25390 4572991..4573422 + 432 WP_017898991.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4523000..4599056 76056


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-61)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6632.83 Da        Isoelectric Point: 11.0174

>T79048 WP_012542177.1 NZ_CP022444:4568211-4568387 [Klebsiella sp. LY]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Download         Length: 177 bp

>T79048 NZ_CP022444:4568211-4568387 [Klebsiella sp. LY]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCAGCTCAAGGGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AACTCGGTATCAAATAA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14959.96 Da        Isoelectric Point: 4.4277

>AT79048 WP_017898984.1 NZ_CP022444:4568435-4568842 [Klebsiella sp. LY]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARLINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIVVH

Download         Length: 408 bp

>AT79048 NZ_CP022444:4568435-4568842 [Klebsiella sp. LY]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACGGG
AGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCCTGGTAACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCTTGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTGTCTCTTGGTCACAGTACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTGTCG
TCCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q8YTV3

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q9U6R4

References