Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3733923..3734542 Replicon chromosome
Accession NZ_CP022444
Organism Klebsiella sp. LY

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CE636_RS21180 Protein ID WP_002892050.1
Coordinates 3733923..3734141 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CE636_RS21185 Protein ID WP_002892066.1
Coordinates 3734168..3734542 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CE636_RS21150 3729966..3730229 + 264 WP_004204754.1 type B 50S ribosomal protein L31 -
CE636_RS21155 3730229..3730369 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CE636_RS21160 3730366..3731064 - 699 WP_017898895.1 GNAT family N-acetyltransferase -
CE636_RS21165 3731165..3732622 + 1458 WP_017898894.1 PLP-dependent aminotransferase family protein -
CE636_RS21170 3732591..3733061 - 471 WP_017898893.1 YlaC family protein -
CE636_RS28905 3733104..3733226 + 123 WP_032426076.1 hypothetical protein -
CE636_RS21175 3733198..3733764 - 567 WP_004204750.1 maltose O-acetyltransferase -
CE636_RS21180 3733923..3734141 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CE636_RS21185 3734168..3734542 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CE636_RS21190 3735027..3738173 - 3147 WP_017898892.1 multidrug efflux RND transporter permease subunit -
CE636_RS21195 3738196..3739389 - 1194 WP_004204747.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T79046 WP_002892050.1 NZ_CP022444:c3734141-3733923 [Klebsiella sp. LY]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T79046 NZ_CP022444:c3734141-3733923 [Klebsiella sp. LY]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT79046 WP_002892066.1 NZ_CP022444:c3734542-3734168 [Klebsiella sp. LY]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT79046 NZ_CP022444:c3734542-3734168 [Klebsiella sp. LY]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References