Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 86101..86626 Replicon plasmid unnamed1
Accession NZ_CP022442
Organism Klebsiella sp. LY

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag CE636_RS02410 Protein ID WP_013023785.1
Coordinates 86101..86406 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag CE636_RS02415 Protein ID WP_001568025.1
Coordinates 86408..86626 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CE636_RS02380 81788..82414 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
CE636_RS02385 82534..82713 + 180 WP_006788217.1 hypothetical protein -
CE636_RS02390 83177..83971 - 795 WP_004197635.1 site-specific integrase -
CE636_RS02395 84169..85185 - 1017 WP_017899884.1 hypothetical protein -
CE636_RS02400 85196..85510 - 315 WP_053389906.1 hypothetical protein -
CE636_RS02405 85537..85932 - 396 WP_017899885.1 hypothetical protein -
CE636_RS02410 86101..86406 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
CE636_RS02415 86408..86626 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CE636_RS02430 87257..87454 + 198 WP_017899886.1 hypothetical protein -
CE636_RS02435 87444..87734 - 291 WP_013023783.1 hypothetical protein -
CE636_RS02440 87731..88858 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
CE636_RS02445 88892..90484 - 1593 WP_015344964.1 hypothetical protein -
CE636_RS02450 90691..91470 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / blaCTX-M-15 - 1..95324 95324


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T79034 WP_013023785.1 NZ_CP022442:c86406-86101 [Klebsiella sp. LY]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T79034 NZ_CP022442:c86406-86101 [Klebsiella sp. LY]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT79034 WP_001568025.1 NZ_CP022442:c86626-86408 [Klebsiella sp. LY]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT79034 NZ_CP022442:c86626-86408 [Klebsiella sp. LY]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References