Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 4162706..4163351 Replicon chromosome
Accession NZ_CP022437
Organism Virgibacillus necropolis strain LMG 19488

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag CFK40_RS19815 Protein ID WP_089534079.1
Coordinates 4162706..4163056 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag CFK40_RS19820 Protein ID WP_168927243.1
Coordinates 4163061..4163351 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFK40_RS19780 4158088..4158879 - 792 WP_089534073.1 RNA polymerase sigma factor SigB -
CFK40_RS19785 4158851..4159324 - 474 WP_089534074.1 anti-sigma B factor RsbW -
CFK40_RS19790 4159327..4159656 - 330 WP_089534075.1 STAS domain-containing protein -
CFK40_RS19795 4159759..4160754 - 996 WP_089534463.1 PP2C family protein-serine/threonine phosphatase -
CFK40_RS19800 4160885..4161286 - 402 WP_089534076.1 anti-sigma regulatory factor -
CFK40_RS19805 4161290..4161646 - 357 WP_089534077.1 STAS domain-containing protein -
CFK40_RS19810 4161651..4162520 - 870 WP_089534078.1 RsbT co-antagonist protein RsbRA -
CFK40_RS19815 4162706..4163056 - 351 WP_089534079.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CFK40_RS19820 4163061..4163351 - 291 WP_168927243.1 antitoxin Antitoxin
CFK40_RS19825 4163516..4164655 - 1140 WP_089534081.1 alanine racemase -
CFK40_RS19830 4164819..4165145 + 327 WP_161493910.1 STAS domain-containing protein -
CFK40_RS19835 4165230..4166249 - 1020 WP_089534083.1 outer membrane lipoprotein carrier protein LolA -
CFK40_RS19840 4166859..4167005 - 147 WP_089534084.1 hypothetical protein -
CFK40_RS19845 4167182..4167538 - 357 WP_089534085.1 holo-ACP synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13034.24 Da        Isoelectric Point: 9.5926

>T79033 WP_089534079.1 NZ_CP022437:c4163056-4162706 [Virgibacillus necropolis]
MIVQRGEVYFADLSPVVGSEQGGIRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKRYGFDRNSVILLEQI
RTLDKQRLTDKITKLDNEMMKKIDQALEISLGLKDM

Download         Length: 351 bp

>T79033 NZ_CP022437:c4163056-4162706 [Virgibacillus necropolis]
TTGATCGTTCAACGTGGTGAAGTATATTTCGCTGACTTATCCCCTGTGGTAGGATCAGAACAGGGAGGTATACGTCCAGT
ATTGATCTTACAAAATGATATCGGAAATAGGTTTAGTCCTACAGTAATTGTTGCAGCAATTACTGCTCAAATTCAAAAAG
CAAAGCTACCAACACATGTTGAGATTAATGCGAAGCGGTATGGGTTTGATCGTAATTCTGTGATATTGCTAGAACAAATT
CGGACACTCGATAAACAAAGACTAACAGATAAGATTACAAAGTTAGACAATGAAATGATGAAAAAAATAGATCAAGCATT
GGAAATAAGTCTTGGACTTAAAGACATGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 11202.67 Da        Isoelectric Point: 4.3640

>AT79033 WP_168927243.1 NZ_CP022437:c4163351-4163061 [Virgibacillus necropolis]
MVLSESLQEILVRLPKNLLNEVDGLMKYDNRDLSDFICQATKNYLEQKKYDHIQQFRESMQKGYEEMARINLTIASEAFQ
AEEEAENTLERSVIGV

Download         Length: 291 bp

>AT79033 NZ_CP022437:c4163351-4163061 [Virgibacillus necropolis]
ATGGTTTTGTCAGAGAGCTTACAGGAGATTTTAGTACGATTACCAAAAAACCTATTAAATGAGGTGGATGGACTAATGAA
GTATGATAATAGAGATCTTAGCGATTTTATCTGTCAGGCAACAAAGAATTATTTAGAGCAAAAGAAATATGACCATATTC
AACAATTTCGAGAATCCATGCAAAAAGGGTATGAGGAAATGGCTCGAATTAATCTAACAATTGCCTCGGAGGCTTTTCAA
GCTGAAGAGGAGGCTGAAAACACCCTTGAGCGTTCTGTGATCGGGGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References